The effect of plasticisers on “sulphate supply” enzymes

None of the compounds tested was cytotoxic in TE 671 cells, however, cell proliferation was significantly increased with 0.005-0.5 μM dioctyl phthalate, diisodecyl phthalate (DIP) and butylbenzyl phthalate (P < 0.05, n = 4). Real time RT-PCR showed dose-dependent decreases in steady-state mRNA levels of all the enzymes studied (P < 0.05, n = 4) with 0.005-0.5 μM octylphenol, bis (2-ethylhexyl) phthalate and DIP treatment. Endocrine disrupting effects of some plasticisers may be a consequence of modulation of expression of enzymes supplying PAPS for hormone sulphation.