کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9914895 | 1551014 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential regulation of corticotropin-releasing factor receptor type 1 (CRF1 receptor) mRNA via protein kinase A and mitogen-activated protein kinase pathways in rat anterior pituitary cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
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چکیده انگلیسی
Corticotropin-releasing factor (CRF) receptor type 1 (CRF1 receptor) mRNA levels are down-regulated by CRF via the cyclic AMP-protein kinase A (PKA) pathway. In this study, we focused on the involvement of both the mitogen-activated protein (MAP) kinase pathway and PKA in this regulation. Real-time PCR (RT-PCR) revealed that a MAP kinase, extracellular signal-regulated kinases 1/2, pathway was also involved in the down-regulation of CRF1 receptor mRNA levels by CRF in the rat anterior pituitary (AP). Down-regulation of CRF1 receptor mRNA levels was caused by a post-transcriptional system such as mRNA degradation, as incubation with CRF significantly decreased the half-life of CRF1 receptor mRNA. Furthermore, pre-treatment with a PKA inhibitor completely blocked CRF-induced CRF1 receptor mRNA destabilization, while pre-treatment with an extracellular signal-regulated kinases 1/2 inhibitor had no inhibitory effect. These results suggested that in the rat AP, down-regulation of CRF1 receptor mRNA levels is caused by mRNA degradation via PKA, but not by the MAP kinase pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Endocrinology - Volume 243, Issues 1â2, 24 November 2005, Pages 74-79
Journal: Molecular and Cellular Endocrinology - Volume 243, Issues 1â2, 24 November 2005, Pages 74-79
نویسندگان
Takako Moriyama, Kazunori Kageyama, Yoko Kasagi, Yasumasa Iwasaki, Takeshi Nigawara, Satoru Sakihara, Toshihiro Suda,