Identification and molecular characterization of three GnRH ligands and five GnRH receptors in the spotted green pufferfish

Gonadotropin-releasing hormone (GnRH) is thought to have diverse physiological functions. Understanding regulatory mechanisms of GnRH functions requires detailed knowledge of gene expressions of both GnRH ligands and receptors in a single species. This report concerns identification and molecular characterization of GnRH ligands and receptors in the spotted green pufferfish Tetraodon nigroviridis. It was identified that the pufferfish possessed three types of GnRH ligands and five types of GnRH receptors. All types of ligands and receptors showed different expression patterns, and were widely expressed both inside and outside the brain. Gonads expressed all the ligand and receptor subtypes. Two of five receptor subtypes could not be detected in the pituitary gland of reproductively active individuals, suggesting the existence of novel GnRH systems independent of hypothalamic-pituitary-gonadal axis. Alternative splicing was also observed for some receptor subtypes. The present results indicate that diversified gene expressions combined with molecular diversity contribute to the functional diversity of GnRH.