کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9921316 | 1559212 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Endothelial mechanisms underlying responses to acetylcholine in the horse deep dorsal penile vein
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب سلولی و مولکولی
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چکیده انگلیسی
This study evaluates the mechanisms underlying endothelium-dependent responses to acetylcholine in horse deep dorsal penile veins. Acetylcholine-induced relaxation was abolished by endothelium removal, the soluble guanylyl cyclase-inhibitor, and the nitric oxide (NO) synthase inhibitors. Acetylcholine-induced relaxation was inhibited by high K+ concentrations and blockade of large-conductance Ca2+-activated potassium (BKCa) channels, and voltage-dependent potassium (Kv) channels. Relaxations were unaffected by a small-conductance KCa (SKCa) channel blocker, or an ATP-sensitive potassium (KATP) channel blocker. Relaxation in response to a NO donor was unaffected by KCa channel blockers, but inhibited by high K+ concentrations and a Kv channel blocker. In the presence of a NO synthase inhibitor, acetylcholine-induced contractions were inhibited by a cyclooxygenase blocker and abolished by endothelial removal. The contractile response was competitively inhibited by muscarinic receptor antagonists, high affinity M1 and M3 antagonists, while the M2 antagonist had no effect. The pharmacological profile suggests that acetylcholine contraction is mediated by muscarinic M1 receptors. Our findings indicate that acetylcholine-induced relaxation in the horse deep dorsal penile vein is essentially mediated by NO, acting via the cGMP-dependent pathway and opening of K+ channels. The contraction elicited by acetylcholine is prostanoid-mediated and induced by endothelial muscarinic M1 receptor activation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmacology - Volume 515, Issues 1â3, 16 May 2005, Pages 150-159
Journal: European Journal of Pharmacology - Volume 515, Issues 1â3, 16 May 2005, Pages 150-159
نویسندگان
Ana Cristina MartÃnez, Dolores Prieto, Medardo Hernández, Luis Rivera, Paz Recio, Albino GarcÃa-Sacristán, Sara Benedito,