کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9921550 1559227 2005 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enhancement of benzodiazepine binding sites following chronic treatment with flumazenil
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
Enhancement of benzodiazepine binding sites following chronic treatment with flumazenil
چکیده انگلیسی
The aim of this study was to improve our knowledge of the mechanisms leading to adaptive changes in γ-aminobutyric acidA (GABAA) receptors following chronic drug treatment. Exposure (48 h) of human embryonic kidney (HEK 293) cells stably expressing recombinant α1β2γ2S GABAA receptors to the antagonist of benzodiazepine binding sites, flumazenil (5 μM), enhanced the maximum number (Bmax) and the equilibrium dissociation constant (Kd) of [3H]flunitrazepam binding sites. The flumazenil-induced enhancement in Bmax was potentiated by GABA (50 μM) and reduced by the GABAA receptor antagonist, bicuculline (100 μM). Flumazenil-induced enhancement in Kd was affected by neither of these treatments. GABA (50 μM) enhanced the density of [3H]flunitrazepam binding sites, and this enhancement was greater in the presence of diazepam (1 μM). The results suggest that chronic flumazenil treatment up-regulates in a bicuculline-sensitive manner benzodiazepine binding sites at stably expressed GABAA receptors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmacology - Volume 507, Issues 1–3, 10 January 2005, Pages 7-13
نویسندگان
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