کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9955311 | 1569759 | 2018 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of novel and conserved microRNA and their expression in the gray mouse lemur, Microcebus murinus, a primate capable of daily torpor
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کلمات کلیدی
RT-PCRKEGGERKAMPKJnkAMP-activated protein kinase - AMP-پروتئین کیناز فعال شده استc-Jun N-terminal kinase - C-Jun N-terminal kinaseMAPK - MAPKRAC-alpha serine/threonine-protein kinase - RAC-alpha serine / ترئونین-پروتئین کینازAkt - آکتMetabolic rate depression - افسردگی متابولیکTorpor - تورپورSra - خانمKyoto Encyclopedia of Genes and Genomes - دایره المعارف ژنتیک ژن ها و ژنوم کیوتوBody temperature - دمای بدنSequence Read Archive - دنباله آرشیو را بخوانیدreverse transcription - رونویسی معکوسEnvironmental adaptation - سازگاری زیست محیطیMicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNAPrimate - نخستیسانان، نخستیها، پریمات هاGene ontology - هستیشناسی ژنیreal-time polymerase chain reaction - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
MicroRNA (miRNA) are endogenous small noncoding RNA gene products, on average 22â¯nt long, that play important regulatory roles in mediating gene expression by binding to and targeting mRNAs for degradation or translational repression. In this paper we identify both novel and conserved miRNA sequences present in the genome of the gray mouse lemur, Microcebus marinus. In total, 122 conserved and 44 novel miRNA were identified with high confidence from the lemur genome (Mmur_2.0) and were used for expression analysis. All conserved and novel miRNA were subjected to relative quantification by RT-qPCR in liver samples from control and torpid lemurs. A total of 26 miRNA (16 conserved and 10 novel) showed increased levels during primate torpor, whereas 31 (30 conserved and 1 novel) decreased. Additional in silico mapping of the predicted mRNA targets of torpor-responsive mature miRNA suggested that miRNA that increased during torpor were collectively involved in cell development and survival pathways, while miRNA that decreased were enriched in targeting immune function. Overall, the study suggests new regulatory mechanisms of primate torpor via miRNA action.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 677, 30 November 2018, Pages 332-339
Journal: Gene - Volume 677, 30 November 2018, Pages 332-339
نویسندگان
K.K. Biggar, B.E. Luu, C.W. Wu, F. Pifferi, M. Perret, K.B. Storey,