The E104D mutation increases the susceptibility of human triosephosphate isomerase to proteolysis. Asymmetric cleavage of the two monomers of the homodimeric enzyme
Keywords: HsTIM; human triosephosphate isomerase; DTNB; 5,5â²-dithio-bis (2-nitrobenzoic acid); CAP; 3-chloroacetol phosphate; PVDF; polyvinylidene difluoride; ANS; 8-anilinonaphthalene 1-sulphonate; Triosephosphate isomerase; TIM deficiency; X-ray crystallography