Keywords: نشانگر وابستگی; Affinity tag; Tandem tag; Tag removal; Tobacco etch virus protease; Monoclonal antibody; Sandwich ELISA;
مقالات ISI نشانگر وابستگی (ترجمه نشده)
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Keywords: نشانگر وابستگی; Fusion protein; Expression; Solubility; Purification; Solubility-enhancing tag; Affinity tag; R9AP; RGS9-1 anchor protein; CMC; critical micellar concentration; GST; glutathione S-transferase; IMAC; immobilized metal affinity chromatography; IPTG; 1-Propy
Keywords: نشانگر وابستگی; Heparin; Affinity tag; Single-step; Recombinant proteins and protein purificationHB, heparin-binding; GST, glutathione-S-transferase; GSH, reduced glutathione; IPTG, isopropyl thiogalactopyranoside; PMSF, phenyl methyl sulfonyl fluoride
Keywords: نشانگر وابستگی; Antibody binding protein; Affinity tag; Immunoassay; Avidity effect; Linker engineering
Keywords: نشانگر وابستگی; Protein purification; Fusion proteins; Green fluorescent protein; Synthetic ligands; Affinity tag; Chromatography;
Keywords: نشانگر وابستگی; Purification; Fusion protein; Affinity tag; Competitive assay
Keywords: نشانگر وابستگی; Affinity tag; Tag-removing protease; On-column cleavage; Protein complex; Controlled subunit stoichiometry;
Keywords: نشانگر وابستگی; Affinity tag; Tag-removing protease; Salt tolerance; P1' promiscuity; SUMO; NEDD8;
Efficient sortase-mediated N-terminal labeling of TEV protease cleaved recombinant proteins
Keywords: نشانگر وابستگی; Sortase; Post-labeling purification; TEV; LPXTG; Affinity tag; TEV; Tobacco Etch Virus; EGFR; epidermal growth factor receptor; MSP; membrane scaffold protein; HER; Human Epidermal growth factor Receptor; KD; kinase domain; SrtA; Sortase A; ACP; acyl carr
Production of recombinant proteins in Escherichia coli tagged with the fusion protein CusF3H+
Keywords: نشانگر وابستگی; Fusion protein; Affinity tag; CusF3H+; Recombinant proteins; Escherichia coli; CusF; Cation efflux system protein; CusF3H+; mutant of CusF; GFP; Green Fluorescent Protein; MBP; maltose-binding protein; GST; glutathione S-transferase; IMAC; immobilized-met
Expression and purification of recombinant proteins in Escherichia coli tagged with the metal-binding protein CusF
Keywords: نشانگر وابستگی; Fusion protein; CusF; Affinity tag; Escherichia coliCusF, Cu sensitive free protein; GFP, Green Fluorescent Protein; MBP, maltose-binding protein; GST, glutathione S-transferase; IMAC, immobilized-metal affinity chromatography; AXR3, Auxin-responsive prot
Expression and purification of recombinant proteins in Escherichia coli tagged with a small metal-binding protein from Nitrosomonas europaea
Keywords: نشانگر وابستگی; Fusion protein; Small metal-binding protein; Affinity tag; Escherichia coliSmbP, Small metal-binding protein; IMAC, Immobilized-metal affinity chromatography; MBP, Maltose-binding protein; GST, Glutathione S-transferase; EDTA, Ethylenediaminetetraacetic a
Recombinant protein production data after expression in the bacterium Escherichia coli
Keywords: نشانگر وابستگی; Fusion protein; Affinity tag; Escherichia coli; CusF; GST
Heterologous overproduction of 2[4Fe4S]- and [2Fe2S]-type clostridial ferredoxins and [2Fe2S]-type agrobacterial ferredoxin
Keywords: نشانگر وابستگی; Clostridial ferredoxin; Agrobacterial ferredoxin; heterologous expression; Iron–sulfur cluster; Affinity tag
Tryptophan tags and de novo designed complementary affinity ligands for the expression and purification of recombinant proteins
Keywords: نشانگر وابستگی; Affinity tag; Affinity ligand; Combinatorial chemistry; Ugi reaction; Green fluorescent protein; Recombinant proteins; Affinity chromatography;
Thy1.2 driven expression of transgenic His6-SUMO2 in the brain of mice alters a restricted set of genes
Keywords: نشانگر وابستگی; SUMOylation; Transgenic mice; Affinity tag; Penk1; Gene array;
The Fh8 tag: A fusion partner for simple and cost-effective protein purification in Escherichia coli
Keywords: نشانگر وابستگی; Fh8 tag; Affinity tag; Calcium binding protein; Hydrophobic interaction chromatography; Escherichia coli;
Synthesis of deguelin-biotin conjugates and investigation into deguelin's interactions
Keywords: نشانگر وابستگی; Rotenoid; Deguelin; Natural product; Affinity tag;
The Dac-tag, an affinity tag based on penicillin-binding protein 5
Keywords: نشانگر وابستگی; Affinity tag; Protein purification; Penicillin-binding protein 5; Ampicillin Sepharose;
Affinity purification of protein complexes for analysis by multidimensional protein identification technology
Keywords: نشانگر وابستگی; Quantitative proteomics; Multidimensional protein identification technology; Mass spectrometry; Affinity tag; Protein complex; Normalized spectral abundance factor;
The silica-binding Si-tag functions as an affinity tag even under denaturing conditions
Keywords: نشانگر وابستگی; Affinity purification; Affinity tag; Inclusion body; Intrinsically disordered protein; Silica-binding protein; Si-tag;
The substrate specificity of Metarhizium anisopliae and Bos taurus carboxypeptidases A: Insights into their use as tools for the removal of affinity tags
Keywords: نشانگر وابستگی; Carboxypeptidase; Affinity tag; Polyhistidine tag; His-tag; Protease
An overview of enzymatic reagents for the removal of affinity tags
Keywords: نشانگر وابستگی; Carboxypeptidase; Aminopeptidase; PreScission protease; Affinity tag; Polyhistidine tag; His-tag; TEV protease; Enterokinase; Enteropeptidase; Thrombin; 3C protease; Factor Xa;
Single-step affinity purification of recombinant proteins using the silica-binding Si-tag as a fusion partner
Keywords: نشانگر وابستگی; Affinity purification; Affinity tag; Disordered protein; Silanol group; Silica-binding protein; Si-tag;
Tamavidin, a versatile affinity tag for protein purification and immobilization
Keywords: نشانگر وابستگی; Affinity tag; Avidin; Biotin; Escherichia coli; Immobilization; Purification
Use of proteomics for design of a tailored host cell for highly efficient protein purification
Keywords: نشانگر وابستگی; Protein purification; Immobilized metal affinity chromatography; E. coli host cell protein contamination; Proteomics; Host cell design; GFP; Affinity tag
Molecular cloning, overproduction, purification and biochemical characterization of the p39 nsp2 protease domains encoded by three alphaviruses
Keywords: نشانگر وابستگی; Protease; Affinity tag; Fusion protein; Maltose-binding protein; Venezuelan equine encephalitis virus; Sindbis virus; Semliki forest virus; Nonstructural protein 2;
Purification and immobilization of recombinant variants of Brevundimonas diminuta glutaryl-7-aminocephalosporanic acid acylase expressed in Escherichia coli cells
Keywords: نشانگر وابستگی; Glutaryl-7-aminocephalosporanic acid acylase; Chitin-binding domain; Affinity tag; Fusion protein
Fluorescence labeling of a cytokine with desthiobiotin-tagged fluorescent puromycin
Keywords: نشانگر وابستگی; affinity tag; cytokine; desthiobiotin tag; fluorescence labeling method; fluorescence microscopy; puromycin; tandem affinity purification
Characterization of a cellulose binding domain from Clostridium cellulovorans endoglucanase-xylanase D and its use as a fusion partner for soluble protein expression in Escherichia coli
Keywords: نشانگر وابستگی; Cellulose binding domain; EngD; Solubility; Immobilization; Protein fusion; Purification; Amorphous cellulose; Affinity tag
A double epitope tag for quantification of recombinant protein using fluorescence resonance energy transfer
Keywords: نشانگر وابستگی; Rare earth cryptate; Homogeneous time-resolved fluorescence; Affinity tag; Protein expression; Protein purification; Recombinant protein; Immunoassay
A novel recombinant system for functional expression of myonecrotic snake phospholipase A2 in Escherichia coli using a new fusion affinity tag
Keywords: نشانگر وابستگی; Affinity tag; Chaperones; Congerin; Escherichia coli; Galectin; Microbacterium liquefaciens protease; Myotoxin; Phospholipase A2; Protobothrops flavoviridis; Recombinant expression; Snake venome; Thrombin
One-step purification of insoluble hydantoinase overproduced in Escherichia coli
Keywords: نشانگر وابستگی; Hydantoinase; Intein; Affinity tag; Artificial oil body; Oleosin
Search for a dextransucrase minimal motif involved in dextran binding
Keywords: نشانگر وابستگی; DSR-S, Leuconostoc mesenteroides NRRL B512-F dextransucrase; GBD, glucan-binding domain; GH, glycoside-hydrolase; His, polyhistidine tagGlucan-binding domain; Dextransucrase; Affinity tag; Protein purification
Mutations that alter the equilibrium between open and closed conformations of Escherichia coli maltose-binding protein impede its ability to enhance the solubility of passenger proteins
Keywords: نشانگر وابستگی; Maltose-binding protein; Solubility enhancer; Affinity tag; Fusion protein; Solubility tag;
Esterase 2 from Alicyclobacillus acidocaldarius as a reporter and affinity tag for expression and single step purification of polypeptides
Keywords: نشانگر وابستگی; Esterase; Reporter enzyme; Affinity tag; Trifluoromethyl-alkyl-ketone
Human glutaredoxin 2 affinity tag for recombinant peptide and protein purification
Keywords: نشانگر وابستگی; Affinity tag; Protein purification; Affinity chromatography; Fusion protein; Glutathione; Glutathione-S-transferase; Thioredoxin
Solubility-enhancing proteins MBP and NusA play a passive role in the folding of their fusion partners
Keywords: نشانگر وابستگی; Maltose-binding protein; NusA; Solubility enhancer; Solubility tag; Affinity tag; Fusion protein; Inclusion bodies; Passenger protein
Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins
Keywords: نشانگر وابستگی; Affinity purification; Affinity tag; His-tag; IMAC; Recombinant protein; Protein purification; Tag removal; Endoproteases; Exopeptidases; Aminopeptidases; Therapeutic protein purification
The esterase from Alicyclobacillus acidocaldarius as a reporter enzyme and affinity tag for protein biosynthesis
Keywords: نشانگر وابستگی; In vitro translation; Esterase; Reporter enzyme; Affinity tag; Protein engineering
Self-assembled luminescent CdSe-ZnS quantum dot bioconjugates prepared using engineered poly-histidine terminated proteins
Keywords: نشانگر وابستگی; Quantum dot; Histidine tag; Affinity tag; Bioconjugation; Single chain antibody; scFv; TNT; Immunoassay;