کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
22418 43276 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fluorescence labeling of a cytokine with desthiobiotin-tagged fluorescent puromycin
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Fluorescence labeling of a cytokine with desthiobiotin-tagged fluorescent puromycin
چکیده انگلیسی

Fluorescence labeling of a cytokine at a specific site is required for observing cytokine-receptor interactions in living cells at the single-molecule level. Here, we demonstrated the C-terminus-specific fluorescence labeling of histidine-tagged thrombopoietin (TPO), a ligand for Mpl, with desthiobiotin-tagged fluorescent puromycin. Fluorescent TPO, purified by tandem affinity purification, stimulated the proliferation of Mpl-expressing cells. Within 10 min of its addition, fluorescent TPO was found to be diffusely distributed on the cell membranes of Mpl-expressing cells, and gradually accumulated to form fluorescent spots. This method is applicable for studying the spatial and temporal distributions of cytokines in individual living cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 105, Issue 3, March 2008, Pages 238–242
نویسندگان
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