|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|19973||43151||2016||5 صفحه PDF||سفارش دهید||دانلود کنید|
• Recombinant PDC gene was stably expressed by GP7.
• PDC gene expression of GP7 improved PDC productivity and ethanol production.
• The direct production of ethanol from wood was improved by using GP7.
To improve ethanol production by Phanerochaete sordida YK-624, the pyruvate decarboxylase (PDC) gene was cloned from and reintroduced into this hyper lignin-degrading fungus; the gene encodes a key enzyme in alcoholic fermentation. We screened 16 transformant P. sordida YK-624 strains that each expressed a second, recombinant PDC gene (pdc) and then identified the transformant strain (designated GP7) with the highest ethanol production. Direct ethanol production from hardwood was 1.41 higher with GP7 than with wild-type P. sordida YK-624. RT-PCR analysis indicated that the increased PDC activity was caused by elevated recombinant pdc expression. Taken together, these results suggested that ethanol production by P. sordida YK-624 can be improved by the stable expression of an additional, recombinant pdc.
Journal: Journal of Bioscience and Bioengineering - Volume 122, Issue 1, July 2016, Pages 17–21