Keywords: AUC، ultracentrifugation تحلیلی; AUC, analytical ultracentrifugation; hLF, human lactoferrin; Ig, immunoglobulin; ITC, isothermal titration calorimetry; MC-MSSV, mass-conservation multisignal sedimentation velocity; MSSV, multisignal sedimentation velocity; Tp34, the recombinant gene pro
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NADH oxidase and alkyl hydroperoxide reductase subunit C (peroxiredoxin) from Amphibacillus xylanus form an oligomeric assembly
Keywords: AUC، ultracentrifugation تحلیلی; Nox, NADH oxidase; AhpC (Prx), peroxiredoxin; AS, ammonium sulfate; SPR, surface plasmon resonance; DLS, dynamic light scattering; AUC, analytical ultracentrifugation; SAXS, small-angle X-ray scatteringAmphibacillus xylanus; NADH oxidase; AhpC (Prx); Prot
The C-terminal region of the human p23 chaperone modulates its structure and function
Keywords: AUC، ultracentrifugation تحلیلی; AUC, analytical ultracentrifugation; CD, circular dichroism; DSC, differential scanning calorimetry; ƒ/ƒ0, frictional ratio; MM, molecular mass; Rs, Stokes radius; s, sedimentation coefficient; s20,w, s at standard conditions; s020,w, s20,w at 0 mg/mL of
The apicomplexan glideosome and adhesins – Structures and function
Keywords: AUC، ultracentrifugation تحلیلی; Pf, Plasmodium falciparum; Pb, Plasmodium berghei; Pv, Plasmodium vivax; Py, Plasmodium yoelii; Pk, Plasmodium knowlesi; Tg, Toxoplasma gondii; ACT1, actin 1; AMA1, apical membrane antigen 1; AUC, analytical ultracentrifugation; DARC, Duffy antigen/chemok
Identification of a surface glutamine residue (Q64) of Escherichia coli EntA required for interaction with EntE
Keywords: AUC، ultracentrifugation تحلیلی; AUC, analytical ultracentrifugation; CD, circular dichroism; NRPS, non-ribosomal peptide synthesis; DHB, 2,3-dihydroxybenzoic acid; Fur, ferric uptake regulator; SDS–PAGE, sodium dodecylsulfate–polyacrylamide gel electrophoresis; TCEP, Tris(2-carboxyethyl
Nanotherapeutics-Product Development Along the “Nanomaterial” Discussion
Keywords: AUC، ultracentrifugation تحلیلی; nanotechnology; materials science; drug delivery systems; regulatory science; EMA; US FDA; toxicity; nanoparticles; regulations; government; ADME, absorption distribution metabolism elimination; AFM, atomic force microscopy; AUC, analytical ultracentrifug
Structural basis for recognition of the type VI spike protein VgrG3 by a cognate immunity protein
Keywords: AUC، ultracentrifugation تحلیلی; T6SS, the type VI secretion system; VgrG, valine–glycine repeat protein G; TsaB, the type VI secretion antitoxin B protein; AUC, analytical ultracentrifugation; SPR, surface plasmon resonance; SAXS, small-angle X-ray scatteringThe type VI secretion system
“Polymeromics”: Mass spectrometry based strategies in polymer science toward complete sequencing approaches: A review
Keywords: AUC، ultracentrifugation تحلیلی; 2D-LC, two-dimensional liquid chromatography; AUC, analytical ultracentrifugation; APCI, atmospheric pressure chemical ionization; CCE, characteristic collision energy; CID, collision-induced dissociation; CRP, controlled radical polymerization; ECD, elec
A DNA Mimic: The Structure and Mechanism of Action for the Anti-Repressor Protein AbbA
Keywords: AUC، ultracentrifugation تحلیلی; SELC, size-exclusion liquid chromatography; ITC, isothermal titration calorimetry; HSQC, heteronuclear single quantum coherence; NOE, nuclear Overhauser enhancement; NOESY, NOE spectroscopy; AUC, analytical ultracentrifugation; PCA, principal components a
Non-canonical residues of the marginally stable monomeric ubiquitin conjugase from goldfish are involved in binding to the C terminus of Ring 1B
Keywords: AUC، ultracentrifugation تحلیلی; ANS, 1-anilino-8-naphtalene sulfonate; AUC, analytical ultracentrifugation; C-Ring 1B, the C terminus of murine Ring 1B, comprising residues 227–334 of the intact Ring 1B; CD, circular dichroism; DOSY, diffusion ordered spectroscopy; DSC, differential sca
Analytical aspects of biosimilarity issues of protein drugs
Keywords: AUC، ultracentrifugation تحلیلی; AC, affinity chromatography; ACE, affinity capillary electrophoresis; AUC, analytical ultracentrifugation; CD, circular dichroism; CDRs, complementarity determining regions; CE, capillary electrophoresis; CEC, capillary electrophoresis chromatography; CGE
Protein Kinase Domain of CTR1 from Arabidopsis thaliana Promotes Ethylene Receptor Cross Talk
Keywords: AUC، ultracentrifugation تحلیلی; CTR1, constitutive triple response 1; MAPKKK, mitogen-activated protein kinase kinase kinase; WT, wild type; MS, mass spectrometry; MS/MS, tandem mass spectrometry; AUC, analytical ultracentrifugation; ES, enzyme–substrate; PDB, Protein Data Bankethylene
Structural, Bioinformatic, and In Vivo Analyses of Two Treponema pallidum Lipoproteins Reveal a Unique TRAP Transporter
Keywords: AUC، ultracentrifugation تحلیلی; AUC, analytical ultracentrifugation; β-OG, n-octyl-β-d-glucoside; HA, hydrophobic antechamber; PDB, Protein Data Bank; PEG, polyethylene glycol; RT-PCR, reverse transcription PCR; SBP, substrate-binding protein; SEC, size-exclusion chromatography; SV, sed
Physical Chemistry of Polyglutamine: Intriguing Tales of a Monotonous Sequence
Keywords: AUC، ultracentrifugation تحلیلی; polyQ, polyglutamine; HD, Huntington's disease; htt, huntingtin; FCS, fluorescence correlation spectroscopy; httNT, htt 17-amino acid N-terminus; AUC, analytical ultracentrifugation; GFP, green fluorescent protein; FTIR, Fourier transform infrared spectro
Sedimentation velocity to characterize surfactants and solubilized membrane proteins
Keywords: AUC، ultracentrifugation تحلیلی; AcrB, acriflavine resistance protein B from Escherichia coli; AUC, analytical ultracentrifugation; CMC, critical micelle concentration; pd complex, protein–detergent complex; SE, Sedimentation equilibrium; SV, Sedimentation velocity analytical ultracentri
The Nonlinear Structure of the Desmoplakin Plakin Domain and the Effects of Cardiomyopathy-Linked Mutations
Keywords: AUC، ultracentrifugation تحلیلی; SR, spectrin repeat; PKP, plakophilin; ARVC, arrhythmogenic right ventricular cardiomyopathy; SH3, Src homology 3; AUC, analytical ultracentrifugation; SAXS, small-angle X-ray scattering; GST, glutathione S-transferase; MBP, maltose binding proteindesmopl
Activation of the Zymogen to Urokinase-Type Plasminogen Activator Is Associated with Increased Interdomain Flexibility
Keywords: AUC، ultracentrifugation تحلیلی; ATF, amino-terminal fragment; AUC, analytical ultracentrifugation; EGF, epidermal growth factor; EOM, ensemble optimization method; SAXS, small-angle X-ray scattering; uPA, urokinase-type plasminogen activator; uPAR, uPA receptor; NSD, normalized spatial
Structural and Thermodynamic Comparison of the Catalytic Domain of AMSH and AMSH-LP: Nearly Identical Fold but Different Stability
Keywords: AUC، ultracentrifugation تحلیلی; DUB, deubiquitinating enzyme; EGFR, epidermal growth factor receptor; GdHCl, guanidine hydrochloride; AUC, analytical ultracentrifugation; MR, molecular replacement; PDB, Protein Data Bank; GST, glutathione S-transferase; SEC, size-exclusion chromatograph
Insights into Metalloregulation by M-box Riboswitch RNAs via Structural Analysis of Manganese-Bound Complexes
Keywords: AUC، ultracentrifugation تحلیلی; NMIA, N-methylisatoic anhydride; AUC, analytical ultracentrifugation; SHAPE, selective 2′-hydroxyl acylation analyzed by primer extension; PDB, Protein Data Bankriboswitch; magnesium–RNA interaction; RNA folding; RNA structure; X-ray crystallography
Evaluating the stoichiometry of macromolecular complexes using multisignal sedimentation velocity
Keywords: AUC، ultracentrifugation تحلیلی; Arp2/3, actin related protein 2–actin related protein 3 complex; AUC, analytical ultracentrifugation; LE, Lamm equation; MSSV, multisignal sedimentation velocity; OD, optical density; SE, sedimentation equilibrium; SV, sedimentation velocity analytical ul
Mapping the Deltex-Binding Surface on the Notch Ankyrin Domain Using Analytical Ultracentrifugation
Keywords: AUC، ultracentrifugation تحلیلی; Dx, Deltex; NICD, Notch intracellular domain; AUC, analytical ultracentrifugation; MASLS, multi-angle static light scattering; SA model, self-association model; SA+ID model, self-association, incompetent dimer model; MBP, maltose binding proteinNotch sign
The Baseplate Wedges of Bacteriophage T4 Spontaneously Assemble into Hubless Baseplate-Like Structure In Vitro
Keywords: AUC، ultracentrifugation تحلیلی; gp, gene product; SDG, sucrose density gradient; 3D, three-dimensional; EM, electron microscopy; SV, sedimentation velocity; AUC, analytical ultracentrifugation; SEC, size-exclusion chromatography; Mw, molecular weightbacteriophage T4; baseplate; assembly
The sedimentation properties of ferritins. New insights and analysis of methods of nanoparticle preparation
Keywords: AUC، ultracentrifugation تحلیلی; AUC, analytical ultracentrifugation; DTT, dithiothreitol; HoSF, horse spleen ferritin; HuHF, recombinant human H-chain ferritin; MOPS, 3-(N-morpholino)propanesulfonic acid; TEM, transmission electron microscopy; D, diffusion coefficient (cm2/s) under the
TolA Modulates the Oligomeric Status of YbgF in the Bacterial Periplasm
Keywords: AUC، ultracentrifugation تحلیلی; AUC, analytical ultracentrifugation; TPR, tetratricopeptide repeat; NTD, N-terminal domain (of YbgF); SEC-MALLS, size-exclusion chromatography–multi-angle laser light scattering; ITC, isothermal titration calorimetry; OM, outer membraneprotein–protein int
Dynamic Interactions between Clathrin and Locally Structured Elements in a Disordered Protein Mediate Clathrin Lattice Assembly
Keywords: AUC، ultracentrifugation تحلیلی; Alexa 488, Alexa Fluor 488 succinimidyl ester; AUC, analytical ultracentrifugation; CBD, clathrin binding domain; CD, circular dichroism; CTD, C-terminal domain; HSQC, heteronuclear single quantum coherence; IDP, intrinsically disordered protein; NOE, nuc
Semi-Rigid Solution Structures of Heparin by Constrained X-ray Scattering Modelling: New Insight into Heparin–Protein Complexes
Keywords: AUC، ultracentrifugation تحلیلی; IdoA, α-l-iduronic acid; VCP, vaccinia complement control protein; AUC, analytical ultracentrifugation; GlcNS, N-suphated glucosamine; PDB, Protein Data Bank; FH, factor H; MIP-1α, macrophage inflammatory protein 1α; SCR, short complement regulatorheparin
α-Helical transmembrane peptides: A “Divide and Conquer” approach to membrane proteins
Keywords: AUC، ultracentrifugation تحلیلی; A2aR, adenosine A2a receptor; ATR-FTIR, attenuated total reflection Fourier transform infrared spectroscopy; AUC, analytical ultracentrifugation; β2AR, β2 adrenergic receptor; BLM, black lipid membrane; CR, conductance recordings (of vesicles, lipid bilay
The FHA-containing protein GarA acts as a phosphorylation-dependent molecular switch in mycobacterial signaling
Keywords: AUC، ultracentrifugation تحلیلی; AUC, analytical ultracentrifugation; CD, circular dichroism; DLS, dynamic light scattering; DSC, differential scanning calorimetry; FHA, Fork-head associated; ITC, isothermal titration calorimetry; MALS, multi-angle light scattering; PAGE, polyacrylamide
Multimeric Interactions between Complement Factor H and Its C3d Ligand Provide New Insight on Complement Regulation
Keywords: AUC، ultracentrifugation تحلیلی; AUC, analytical ultracentrifugation; aHUS, atypical haemolytic uraemic syndrome; CR, complement receptor; FH, factor H; PBS, phosphate-buffered saline; RU, response unit; SCR, short complement regulator; SPR, surface plasmon resonancefactor H; C3d; X-ray
A β-Hairpin Comprising the Nuclear Localization Sequence Sustains the Self-associated States of Nucleosome Assembly Protein 1
Keywords: AUC، ultracentrifugation تحلیلی; NAP-1; NAP1; oligomerization; histone binding; nucleosome assemblyNAP1, nucleosome assembly protein 1; yNAP1, yeast NAP1; NCP, nucleosome core particle; AUC, analytical ultracentrifugation; NLS, nuclear localization sequence; GST, glutathione S-transferas
Conserved Central Domains Control the Quaternary Structure of Type I and Type II Hsp40 Molecular Chaperones
Keywords: AUC، ultracentrifugation تحلیلی; Hsp, heat shock protein; SAXS, small-angle X-ray scattering; AUC, analytical ultracentrifugation; 3D, three-dimensionalHsp40; molecular chaperone; protein folding; quaternary structure; small-angle scattering
Crystal Structure of the Escherichia coli 23S rRNA:m5C Methyltransferase RlmI (YccW) Reveals Evolutionary Links between RNA Modification Enzymes
Keywords: AUC، ultracentrifugation تحلیلی; AdoMet, S-adenosyl-l-methionine; MTase, methyltransferase; RFM, Rossmann-fold methyltransferases; m5C, 5-methylcytosine; SelMet, l-selenomethionine; AUC, analytical ultracentrifugation; PDB, Protein Data Bank; r-protein, ribosomal protein; AdoHcy, S-adeno
Formation of a Stable Oligomer of β-2 Microglobulin Requires only Transient Encounter with Cu(II)
Keywords: AUC، ultracentrifugation تحلیلی; β2m, β-2 microglobulin; DRA, dialysis related amyloidosis; SEC, size-exclusion chromatography; AUC, analytical ultracentrifugation; EPR, electron paramagnetic resonanceβ-2 microglobulin; amyloid; copper; dialysis; aggregation
Oligomeric behavior of the RND transporters CusA and AcrB in micellar solution of detergent
Keywords: AUC، ultracentrifugation تحلیلی; AUC, analytical ultracentrifugation; DDM, n-dodecyl-β-d-maltopyranoside; FC14, n-tetradecylphosphocholine; FTIR, Fourier transform infrared spectroscopy; RND, resistance nodulation and cell divisionRND transporter; Analytical ultracentrifugation; Detergen
Optimization of the Palindromic Order of the TtgR Operator Enhances Binding Cooperativity
Keywords: AUC، ultracentrifugation تحلیلی; AUC, analytical ultracentrifugation; DMS, dimethylsulfate; ECF, extracytoplasmic sigma factor; HTH, helix-turn-helix; ITC, isothermal titration calorimetry; EMSA, electrophoretic mobility shift assays; MDR, multidrug resistance; RND, resistance-nodulation
The Role of a Hydrogen Bonding Network in the Transmembrane β-Barrel OMPLA
Keywords: AUC، ultracentrifugation تحلیلی; AUC, analytical ultracentrifugation; BR, bacteriorhodopsin; C14-SB, 3-(N,N-dimethylmyristyl-ammonio)propanesulfonate; C12-SB, 3-(dodecyldimethyl-ammonio)propanesulfonate; cmc, critical micelle concentration; DEAE, diethylaminoethyl; DTNB, 5,5′-dithiobis(2
The Transcriptional Repressor TtgV Recognizes a Complex Operator as a Tetramer and Induces Convex DNA Bending
Keywords: AUC، ultracentrifugation تحلیلی; AFM, atomic force microscopy; AUC, analytical ultracentrifugation; DMS, dimethylsulfate; IR, inverted repeats; ITC, isothermal titration calorimetry; EMSA, electrophoretic mobility shift assays; wHTH, winged helix-turn-helixTtgV; DNA binding; DNA bending;
Lipid oxidation and amylopectin molecular weight changes occurring during storage of extruded starch samples
Keywords: AUC، ultracentrifugation تحلیلی; Lipid oxidation; Amylopectin; Flow-field-flow fractionation; StorageAF4, asymmetric flow-field-flow fractionation; APcI-MS, atmospheric pressure chemical ionisation-mass spectrometry; AUC, analytical ultracentrifugation; Da, Dalton; DSC, differential scan
Direct Observation of Oligomeric Species formed in the Early Stages of Amyloid Fibril Formation using Electrospray Ionisation Mass Spectrometry
Keywords: AUC، ultracentrifugation تحلیلی; ESI-MS, electrospray ionisation mass spectrometry; β2m, β2-microglobulin; AUC, analytical ultracentrifugation; Thio-T, thioflavin-T; AFM, atomic force microscopyamyloid fibril formation; electrospray ionisation mass spectrometry; beta-2-microglobulin; oli
Alternate Interfaces May Mediate Homomeric and Heteromeric Assembly in the Transmembrane Domains of SNARE Proteins
Keywords: AUC، ultracentrifugation تحلیلی; SNARE; membrane protein; vesicle fusion; thermodynamics; disulfide crosslinkingGpA, glycophorin A; AUC, analytical ultracentrifugation; SNARE, soluble NSF attachment protein receptors; TM, transmembrane domain; CuOP, copper phenanthroline; Syx, syntaxin;
Energetics of Outer Membrane Phospholipase A (OMPLA) Dimerization
Keywords: AUC، ultracentrifugation تحلیلی; thermodynamics; OMPLA; transmembrane protein–protein interactions; beta-barrel; dimerizationASA, accessible surface area; AUC, analytical ultracentrifugation; C14-SB, 3-(N,N-dimethylmyristyl-ammonio)propanesulfonate; C12-SB, 3-(dodecyldimethyl-ammonio)pro
The Co-chaperone p23 Arrests the Hsp90 ATPase Cycle to Trap Client Proteins
Keywords: AUC، ultracentrifugation تحلیلی; molecular chaperone; Hsp90; heat shock; protein folding; p23SPR, surface plasmon resonance; nanoESI-MS, nano-electronspray ionization mass spectrometry; AUC, analytical ultracentrifugation; SAXS, small-angle X-ray scattering; ITC, isothermal titration cal
The dimeric form of flavocytochrome P450 BM3 is catalytically functional as a fatty acid hydroxylase
Keywords: AUC، ultracentrifugation تحلیلی; BM3, flavocytochrome P450 BM3 from Bacillus megaterium; CO, carbon monoxide; AUC, analytical ultracentrifugation; eT, electron transfer; NOS, nitric oxide synthaseCytochrome P450; Oxygenase; Dimerization; Electron transfer; Fatty acids; Enzymology