کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1945903 1053279 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Oligomeric behavior of the RND transporters CusA and AcrB in micellar solution of detergent
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Oligomeric behavior of the RND transporters CusA and AcrB in micellar solution of detergent
چکیده انگلیسی

We have used analytical ultracentrifugation to explore the oligomeric states of AcrB and CusA in micellar solution of detergent. These two proteins belong to the resistance, nodulation and cell division (RND) family of efflux proteins that are involved in multiple drug and heavy metal resistance. Only the structure of AcrB has been determined so far. Although functional RND proteins should assemble as trimers as AcrB does, both AcrB and CusA form a mixture of quaternary structures (from monomer to heavy oligomer) in detergent solution. The distribution of the oligomeric states was studied as a function of different parameters: nature and concentration of the detergent, ionic strength, pH, protein concentration. This pseudo-heterogeneity does not hamper the crystallization of AcrB as a homotrimer.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1768, Issue 6, June 2007, Pages 1567–1573
نویسندگان
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