
Split intein facilitated tag affinity purification for recombinant proteins with controllable tag removal by inducible auto-cleavage
Keywords: DnaBN, N-terminal domain of Ssp DnaB intein; DnaBC, C-terminal domain of Ssp DnaB intein; CBD, chitin binding domain; Trx, thioredoxin; MBP, maltose binding protein; ETI, Erythrina trypsin inhibitor; BNP, brain natriuretic peptideProtein splicing; Protein