کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1204406 965152 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Split intein facilitated tag affinity purification for recombinant proteins with controllable tag removal by inducible auto-cleavage
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Split intein facilitated tag affinity purification for recombinant proteins with controllable tag removal by inducible auto-cleavage
چکیده انگلیسی

Purification tags are robust tools that can be used to purify a variety of target proteins. However, tag removal remains an expensive and significant issue that must be resolved. Based on the affinity and the trans-splicing activity between the two domains of Ssp DnaB split-intein, a novel approach for tag affinity purification of recombinant proteins with controllable tag removal by inducible auto-cleavage has been developed. This system provides a new affinity method and avoids premature splicing of the intein fused proteins expressed in host cells. The affinity matrix can be reused. In addition, this method is compatible with his-tag affinity purification technique. Our methods provide the insights for establishing a novel recombinant protein preparation system.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1218, Issue 18, 6 May 2011, Pages 2553–2560
نویسندگان
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