
Metal ion stimulators of PDE5 cause similar conformational changes in the enzyme as does cGMP or sildenafil
Keywords: PDE; cyclic nucleotide phosphodiesterase; PAGE; polyacrylamide gel electrophoresis; SDS; sodium dodecyl sulfate; KPEM; 10 mM potassium phosphate (pH 6.8) containing 1 mM EDTA and 25 mM β-mercaptoethanol; GAF; cGMP-binding PDE, Anabaenaadenylyl cyclase