Keywords: Condyloma acuminatum; Human papillomavirus; Hansenula polymorpha; Vaccine;
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Keywords: CRISPR-Cas9; Kluyveromyces lactis; Kluyveromyces marxianus; Scheffersomyces stipitis; Yarrowia lipolytica; Hansenula polymorpha; Pichia pastoris; HR; homologous recombination; NHEJ; nonhomologous end-joining; DSB; double strand break; CRISPR; Clustered re
Keywords: CHO; Chinese hamster ovary; DLP1; dynamin-like protein 1; ER; endoplasmic reticulum; FIS1; fission 1; H. polymorpha; Hansenula polymorpha; MFF; mitochondrial fission factor; MAPL; mitochondria-anchored protein ligase; MDV; mitochondria-derived vesicle; mP
Keywords: ARS; autonomously replicating sequence; CEN; centromere; CRISPR; Clustered, Regularly Interspaced, Short Palindromic Repeats; DSB; double strand break; GFP; green fluorescent protein; GRAS; Generally Regarded As Safe; gRNA; guide RNA (for CRISPR); HR; hom
Keywords: In situ product recovery; Hansenula polymorpha; Hollow fibers; Membrane bioreactors; Dissolved Oxygen; Glucose pulse
High-level expression and immunogenicity of porcine circovirus type 2b capsid protein without nuclear localization signal expressed in Hansenula polymorpha
Keywords: Porcine circovirus type 2b; Nuclear localization signal; Hansenula polymorpha; Immunogenicity; Purification; H.polymorpha; Hansenula polymorpha; PCV2b; Porcine circovirus type 2b; NLS; Nuclear localization signal; CP; Capsid protein; VLP; Virus like parti
Synthesis of ethyl (R)-mandelate using recombinant Carboxydothermus hydrogenoformans alcohol dehydrogenase produced by two yeast species
Keywords: Carboxydothermus hydrogenoformans alcohol dehydrogenase; Yeast cell biocatalysis; Ethyl (R)-mandelate; Arxula adeninivorans; Hansenula polymorpha;
Extracellular expression of YlLip11 with a native signal peptide from Yarrowia lipolytica MSR80 in three different yeast hosts
Keywords: Lipase; Yarrowia lipolytica; Arxula adeninivorans; Saccharomyces cerevisiae; Hansenula polymorpha
Scarless gene deletion in methylotrophic Hansenula polymorpha by using mazF as counter-selectable marker
Keywords: Hansenula polymorpha; Escherichia coli; MazF; Counter-selectable marker; Markerless gene deletion; Scarless gene deletion;
Induction, characterization, and heterologous expression of a carotenoid degrading versatile peroxidase from Pleurotus sapidus
Keywords: VP; versatile peroxidase; CCO; carotenoid cleavage oxygenase; TES; trace element solution; ABTS; 2,2â²-azino-di-(3-ethylbenzthiazolin)-6-sulfonic acid; MnP; manganese peroxidase; Pleurotus sapidus; Hansenula polymorpha; Versatile peroxidase; β-Carotene
Pexophagy-linked degradation of the peroxisomal membrane protein Pex3p involves the ubiquitin-proteasome system
Keywords: Pex3p; Ubiquitination; Peroxisome; Pex2p; Pex10p; Proteasome; Protein degradation; Autophagy; Pexophagy; Hansenula polymorpha;
Identification of phosphorylation sites in Hansenula polymorpha Pex14p by mass spectrometry
Keywords: Pex14p; Phosphorylation; Mass spectrometry; Hansenula polymorpha; PeroxisomeAMO, amine oxidase; AOX, alcohol oxidase; PNS, post nuclear supernatant; PTS, peroxisomal targeting signal; PVDF, polyvinylidene difluoride; SDS–PAGE, sodium dodecyl sulfate poly-
Nitrogen-dependent calcineurin activation in the yeast Hansenula polymorpha
Keywords: NCR, nitrogen catabolite repression; ER, endoplasmic reticulum; qRT-PCR, quantitative real time-PCR; NR, nitrate reductaseCalcineurin; Nitrogen; Signalling; Calcium; Yeast; Hansenula polymorpha
Functional and molecular characterization of novel Hansenula polymorpha genes, HpPMT5 and HpPMT6, encoding protein O-mannosyltransferases
Keywords: O-mannosylation; Pmt proteins; Cell wall integrity; Hansenula polymorpha
K+ uptake systems in the yeast Hansenula polymorpha. Transcriptional and post-translational mechanisms involved in high-affinity K+ transporter regulation
Keywords: GFP; green fluorescent protein; WT; wild type; Potassium uptake; TRK1; HAK1; Calcineurin; Endocytosis; Hansenula polymorpha;
Hansenula polymorpha expressed heat shock protein gp96 exerts potent T cell activation activity as an adjuvant
Keywords: gp96; Hansenula polymorpha; Expression; Peptide binding; HBV
Immunogenicity and safety of a novel yeast Hansenula polymorpha-derived recombinant Hepatitis B candidate vaccine in healthy adolescents and adults aged 10–45 years
Keywords: Hepatitis B vaccination; Hepatitis B virus; Hansenula polymorpha; Recombinant hepatits B surface antigen; Vaccination; Aluminum hydroxide adjuvant; Immunogenicity
Pphy—A cell-bound phytase from the yeast Pichia anomala: Molecular cloning of the gene PPHY and characterization of the recombinant enzyme
Keywords: Arxula adeninivorans; Hansenula polymorpha; Pichia anomala; PPHY; Phytase; Yeast
Bioelectrochemical detection of L-lactate respiration using genetically modified Hansenula polymorpha yeast cells overexpressing flavocytochrome b2
Keywords: Genetic modification; Yeast; Hansenula polymorpha; Flavocytochrome b2; Mitochondrial respiratory chain; L-lactate respiration
Improved gene disruption method and Cre-loxP mutant system for multiple gene disruptions in Hansenula polymorpha
Keywords: URA5; Single-stranded DNA; Cre-lox; Multiple gene disruption; Hansenula polymorpha
The major cathepsin L secreted by the invasive juvenile Fasciola hepatica prefers proline in the S2 subsite and can cleave collagen
Keywords: AMC; 7-amino-4-methyl coumarin; FhCL; Fasciola hepatica cathepsin L; Tos; Tosyl; Z; benzyloxycarbonyl; Trematode; Fasciola hepatica NEJ; Cathepsins; Collagenase; Invasion; Hansenula polymorpha;
Expression of bovine follicle-stimulating hormone subunits in a Hansenula polymorpha expression system increases the secretion and bioactivity in vivo
Keywords: Bovine follicle-stimulating hormone; Calnexin; Less-glycosylation; Hansenula polymorpha;
Development of strains of the thermotolerant yeast Hansenula polymorpha capable of alcoholic fermentation of starch and xylan
Keywords: Thermotolerant yeast; Hansenula polymorpha; Heterologous expression; Amylolytic enzymes; Fermentation of starch; Xylan-degrading enzymes; Xylan fermentation
Feeding strategies for the enhanced production of recombinant human serum albumin in the fed-batch cultivation of Hansenula polymorpha
Keywords: Hansenula polymorpha; MOX promoter; Human serum albumin; Fed-batch fermentation; Feeding strategy; Recombinant protein
Hansenula polymorpha maltase gene promoter with sigma 70-like elements is feasible for Escherichia coli-based biotechnological applications: Expression of three genomic levansucrase genes of Pseudomonas syringae pv. tomato
Keywords: Heterologous protein expression; Sigma 70; Levansucrase; Pseudomonas syringae; Hansenula polymorpha; Yeast promoter
The Hansenula polymorpha peroxisomal targeting signal 1 receptor, Pex5p, functions as a tetramer
Keywords: Pex5p; Peroxisome; Membrane transport; Electron microscopy; Hansenula polymorpha
A highly efficient hydrophobic interaction chromatographic absorbent improved the purification of hepatitis B surface antigen (HBsAg) derived from Hansenula polymorpha cell
Keywords: Recombinant HBsAg; Purification; Hydrophobic interaction chromatography; Hansenula polymorpha; Ligand density; Absorbent
Permeabilized cells of flavocytochrome b2 over-producing recombinant yeast Hansenula polymorpha as biological recognition element in amperometric lactate biosensors
Keywords: Flavocytochrome b2; Gene-engineering; Hansenula polymorpha; Permeabilized cells; Amperometric biosensor; l-Lactate
A highly efficient integrated chromatographic procedure for the purification of recombinant hepatitis B surface antigen from Hansenula polymorpha
Keywords: Purification; Recombinant hepatitis B surface antigen; Hansenula polymorpha; Integrated chromatographic process
Role of γ-glutamyltranspeptidase in detoxification of xenobiotics in the yeasts Hansenula polymorpha and Saccharomyces cerevisiae
Keywords: γ-glutamyltranspeptidase; Gene cloning; Yeast; Hansenula polymorpha; Saccharomyces cerevisiae; Xenobiotics; Detoxification;
Defect of vacuolar protein sorting stimulates proteolytic processing of human urokinase-type plasminogen activator in the yeast Hansenula polymorpha
Keywords: Hansenula polymorpha; Protein secretion; Urokinase; Vacuolar protein sorting; Yeast;
Expression of xylA genes encoding xylose isomerases from Escherichia coli and Streptomyces coelicolor in the methylotrophic yeast Hansenula polymorpha
Keywords: xylA gene; Xylose isomerase; Expression in methylotrophic yeast; Hansenula polymorpha; Xylose fermentation;
Expression and purification of human endostatin from Hansenula polymorpha A16
Keywords: Human endostatin; Gene expression; Hansenula polymorpha; Purification; Endothelial cell proliferation; Angiogenesis;
Hansenula polymorpha NMR2 and NMR4, two new loci involved in nitrogen metabolite repression
Keywords: Nitrate assimilation; Nitrogen metabolite repression; NMR genes; Hansenula polymorpha;
The NII2 gene of Hansenula polymorpha is involved in nitrite assimilation
Keywords: Nitrite assimilation; NII2; Hansenula polymorpha; PHO86; Nitrate assimilation; Membrane protein;
Genetic modification of essential fatty acids biosynthesis in Hansenula polymorpha
Keywords: Essential fatty acid; Î6-desaturase; Mucor rouxii; Hansenula polymorpha;
Obstruction of polyubiquitination affects PTS1 peroxisomal matrix protein import
Keywords: Peroxisome; Protein translocation; PTS1 protein import; Ubiquitin; Yeast; Hansenula polymorpha;
New yeast expression platforms based on methylotrophic Hansenula polymorpha and Pichia pastoris and on dimorphic Arxula adeninivorans and Yarrowia lipolytica - A comparison
Keywords: Hansenula polymorpha; Pichia pastoris; Arxula adeninivorans; Yarrrowia lipolytica; Yeast expression platforms;