کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8369147 | 1543033 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High-level expression and immunogenicity of porcine circovirus type 2b capsid protein without nuclear localization signal expressed in Hansenula polymorpha
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
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چکیده انگلیسی
Currently, porcine circovirus type 2b (PCV2b) is the dominant PCV2 genotype causing postweaning multisystemic wasting disease (PMWS) in pigs worldwide. Efforts have been made to develop various recombinant capsid proteins of PCV2b used in vaccines against PCV2b. However, the nuclear localization signal (NLS) of PCV2b capsid protein (CP) was found to inhibit the expression of the whole length capsid protein in E.coli. Here, we expressed a NLS-deleted capsid protein (ÎCP) of PCV2b in Hansenula polymorpha based on the capsid protein of PCV2b strain Y-7 isolated in China. Comparatively, the ÎCP was expressed at a higher level than the CP. The purified ÎCP could self-assemble into virus like particles (VLPs) with similar morphology of the VLPs formed by CP. The purified ÎCP could be recognized by the anti-sera derived from the mice immunized by inactivated PCV2b particles. Furthermore, it induced higher levels of PCV2b specific antibodies than the purified CP in mice. These results showed that the ÎCP, a recombinant PCV2b capsid protein without nuclear localization signal sequence, could be efficiently expressed in Hansenula polymorpha, and used as a candidate antigen for the development of PCV2b vaccines.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biologicals - Volume 51, January 2018, Pages 18-24
Journal: Biologicals - Volume 51, January 2018, Pages 18-24
نویسندگان
Yue Xiao, Peiyan Zhao, Junyang Du, Xin Li, Wenting Lu, Xu Hao, Boqi Dong, Yongli Yu, Liying Wang,