Expression of the functional carbohydrate-binding module (CBM) of human laforin
Keywords: مجازات شامل; Laforin; Carbohydrate-binding module; RGD; Inclusion bodies
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Access to gram scale amounts of functional globular adiponectin from E. coli inclusion bodies by alkaline-shock solubilization
Keywords: مجازات شامل; ACC; acetyl-CoA carboxylase; AdipoR; adiponectin receptor; fAd; full-length adiponectin; gAd; globular adiponectin; HMW, MMW, LMW; high, medium, low molecular weight; Alkaline-shock; Adiponectin; Inclusion bodies; Obesity; Recombinant protein expression;
Construction of synthetic open reading frame encoding human interferon alpha 2b for high expression in Escherichia coli and characterization of its gene product
Keywords: مجازات شامل; Human interferon α2b; High expression; Synthetic ORF; Escherichia coli; Codon optimization; Inclusion bodies;
The nanoscale properties of bacterial inclusion bodies and their effect on mammalian cell proliferation
Keywords: مجازات شامل; Inclusion bodies; Cell growth; Nanoparticles; Stiffness; Wettability; Tissue engineering
Reliable expression and purification of highly insoluble transmembrane domains
Keywords: مجازات شامل; Transmembrane domain; Caveolin; Inclusion bodies; Purification; Expression
Optimization of refolding with simultaneous purification of recombinant human granulocyte colony-stimulating factor from Escherichia coli by immobilized metal ion affinity chromatography
Keywords: مجازات شامل; Recombinant human granulocyte colony-stimulating factor; Inclusion bodies; Protein refolding and purification; Immobilized metal ion affinity chromatography; Protein folding liquid chromatography
Expression and functional characterization of the plant antimicrobial snakin-1 and defensin recombinant proteins
Keywords: مجازات شامل; Antimicrobial proteins; Inclusion bodies; Plant pathogens; Protein solubilization; Recombinant proteins
Expression and purification of the calcium binding photoprotein mitrocomin using ZZ-domain as a soluble partner in E. coli cells
Keywords: مجازات شامل; Coelenterazine; Aequorin; Clytin; Obelin; Inclusion bodies
Purification of truncated and mutated Chemotaxis Inhibitory Protein of Staphylococcus aureus-an anti-inflammatory protein
Keywords: مجازات شامل; Inflammation; Inclusion bodies; Refolding; Purification;
Periplasmically-exported lupanine hydroxylase undergoes transition from soluble to functional inclusion bodies in Escherichia coli
Keywords: مجازات شامل; Protein export; Periplasmic space; Post-translational modifications; Amorphous protein aggregation; Inclusion bodies; Disulphide-bond formation; LH; Quinocytochrome c; Pyrroloquinoline quinine;
Mammalian G-protein-coupled receptor expression in Escherichia coli: I. High-throughput large-scale production as inclusion bodies
Keywords: مجازات شامل; Protein expression; G-protein-coupled receptor; Inclusion bodies; Fermentors
Escherichia coli expression and refolding of E/K-coil-tagged EGF generates fully bioactive EGF for diverse applications
Keywords: مجازات شامل; EGF; E/K-coil; Refolding; Inclusion bodies; Tissue engineering
In vivo aggregation of bovine β-lactoglobulin is affected by Cys at position 121
Keywords: مجازات شامل; β-Lactoglobulin; Inclusion bodies; Aggregation; Escherichia coli; Fourier transform infrared spectroscopy;
Overexpression, on-column refolding and isotopic labeling of Hahellin from Hahella chejuensis, a putative member of the βγ-crystallin superfamily
Keywords: مجازات شامل; βγ-Crystallin; Protein overexpression; Inclusion bodies; On-column refolding; Isotopic labeling; M9 minimal media; HSQC; MALDI-TOF;
A new Gateway® vector and expression protocol for fast and efficient recombinant protein expression in Mycobacterium smegmatis
Keywords: مجازات شامل; Recombinant protein expression; Inclusion bodies; Mycobacterium tuberculosis; Mycobacterium smegmatis; Gateway; Autoinduction media
Membrane docking of an aggregation-prone protein improves its solubilization
Keywords: مجازات شامل; PTS, phosphotransferase system; PIMT, protein isoaspartate methyltransferase; Hsp, heat shock protein; DSP, dithiobis(succinimidylpropionate); SDS, sodium duodecyl sulfate.Protein aggregation; Heat stress; Inclusion bodies; N,N′-diacetylchitobiose phospho
Characterization and functional recovery of a novel antimicrobial peptide (CECdir–CECret) from inclusion bodies after expression in Escherichia coli
Keywords: مجازات شامل; Inclusion bodies; Refolding procedure; Antibacterial peptides; Cecropin
Refolding of endostatin from inclusion bodies using high hydrostatic pressure
Keywords: مجازات شامل; Refolding; High hydrostatic pressure; Endostatin; Inclusion bodies; Aggregate
Expression, purification, and characterization of an immunotoxin containing a humanized anti-CD25 single-chain fragment variable antibody fused to a modified truncated Pseudomonas exotoxin A
Keywords: مجازات شامل; Immunotoxin; Inclusion bodies; Refolding; Vascular leak syndrome
Refolding of recombinant homodimeric malate dehydrogenase expressed in Escherichia coli as inclusion bodies
Keywords: مجازات شامل; Malate dehydrogenase; Expression; Inclusion bodies; Refolding; Folding aids; Artificial chaperone
One-step affinity tag purification of full-length recombinant human AP-1 complexes from bacterial inclusion bodies using a polycistronic expression system
Keywords: مجازات شامل; AP-1; FLAG tag; Hexahistidine tag; Inclusion bodies; Affinity purification
Addition of a polypeptide stretch at the N-terminus improves the expression, stability and solubility of recombinant protein tyrosine phosphatases from Drosophila melanogaster
Keywords: مجازات شامل; Protein tyrosine phosphatase; Expression; Inclusion bodies; Protein solubility; Light scattering;
Optimization of the downstream process for high recovery of rhG-CSF from inclusion bodies expressed in Escherichia coli
Keywords: مجازات شامل; rhG-CSF; Escherichia coli; Inclusion bodies; Cell disruption; Solubilization; Refolding
Immobilized chaperones: A productive alternative to refolding of bacterial inclusion body proteins
Keywords: مجازات شامل; Escherichia coli; Chaperones; Inclusion bodies; Refolding
A screening system for the identification of refolding conditions for a model protein kinase, p38α
Keywords: مجازات شامل; Protein kinases; Protein folding; Folding additives; Inclusion bodies; Refolding; Recombinant protein expression
Cloning and characterization of a recombinant family 5 endoglucanase from Bacillus licheniformis strain B-41361
Keywords: مجازات شامل; Cellulase; Endoglucanase; Bacillus licheniformis; Inclusion bodies
On-column refolding purification and characterization of recombinant human interferon-λ1 produced in Escherichia coli
Keywords: مجازات شامل; Interferon-λ1; On-column refolding; Inclusion bodies; His-tag; Immobilized metal-ion affinity chromatography
Amyloid-linked cellular toxicity triggered by bacterial inclusion bodies
Keywords: مجازات شامل; Inclusion bodies; β-Galactosidase; Amyloid-like structure; Cellular toxicity; Membrane destabilization
Cytosolic Ca2+ regulates protein expression in E. coli through release from inclusion bodies
Keywords: مجازات شامل; Calcium channels; Inclusion bodies; Bacteria; Polyhydroxybutyrate; Aequorin; Calcium in bacteria
Uterine leiomyomas with inclusion bodies: An immunohistochemical and ultrastructural analysis of 12 cases
Keywords: مجازات شامل; Leiomyoma; Uterus; Inclusion bodies; Rhabdoid cells; Skeletal muscle-like cells
Expression, purification, and in vitro refolding of soluble tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)
Keywords: مجازات شامل; sTRAIL; Inclusion bodies; Protein expression; Refolding; Protein purification; CD spectra; Fluorescence spectra
Refolding, purification, and activation of miniplasminogen and microplasminogen isolated from E. coli inclusion bodies
Keywords: مجازات شامل; Miniplasmin; Microplasmin; E. coil expression; Inclusion bodies; Refolding; activation
Fragment complementation for the co-refolding of Thermotoga maritima β-glucosidase by gene splitting at non-homologous region
Keywords: مجازات شامل; β-Glucosidase; Thermotoga maritima; Non-homologous region; Co-refolding; Gene splitting; Inclusion bodies; Fragment complementation
Improved Expression of HLA-A*2402-BSP in Escherichia coli and Its Tetramer Preparation
Keywords: مجازات شامل; tetramer; HLA-A*2402; cytomegalovirus; codon optimization; prokaryotic expression; inclusion bodies;
Overexpression, purification and characterization of the catalytic component of Oplophorus luciferase in the deep-sea shrimp, Oplophorus gracilirostris
Keywords: مجازات شامل; Bioluminescence; Inclusion bodies; Secretion; Refolding; Metal inhibitor; Organic solvent; Leucine-rich repeat
Enteral vaccination of rats against Fasciola hepatica using recombinant cysteine proteinase (cathepsin L1)
Keywords: مجازات شامل; Fasciola hepatica; Oral vaccination; Inclusion bodies; Cysteine proteinase
Current status of technical protein refolding
Keywords: مجازات شامل; Inclusion bodies; Escherichia coli; Refolding; Large scale
Effect of surface histidine mutations and their number on the partitioning and refolding of recombinant human granulocyte-colony stimulating factor (Cys17Ser) in aqueous two-phase systems containing chelated metal ions
Keywords: مجازات شامل; Aqueous two-phase systems; Metal affinity partitioning; Granulocyte-colony stimulating factor; Inclusion bodies; Protein refolding
Inclusion body anatomy and functioning of chaperone-mediated in vivo inclusion body disassembly during high-level recombinant protein production in Escherichia coli
Keywords: مجازات شامل; Inclusion bodies; Disaggregation; Chaperones; Recombinant protein production
Confronting high-throughput protein refolding using high pressure and solution screens
Keywords: مجازات شامل; Inclusion bodies; Aggregation; Misfolding; Solubilization; Protein recovery; Protein refolding; Small molecules assisted refolding; Fractional factorial screen; High hydrostatic pressure; Automation; High-throughput; Structural genomics
Protein activity in bacterial inclusion bodies correlates with predicted aggregation rates
Keywords: مجازات شامل; Inclusion bodies; Recombinant protein expression; Protein aggregation; Protein folding; Escherichia coli
Expression, purification, and renaturation of bone morphogenetic protein-2 from Escherichia coli
Keywords: مجازات شامل; BMP-2; Escherichia coli; Inclusion bodies; Refolding; Heparin column
Neuronal inclusions in sporadic motor neuron disease are negative for alpha-synuclein
Keywords: مجازات شامل; Amyotrophic lateral sclerosis; Inclusion bodies; α-Synuclein; Immunocytochemistry
Expression, purification and refolding of the phosphatase domain of protein phosphatase 1 (Ppt1) from Saccharomyces cerevisiae
Keywords: مجازات شامل; Protein folding; Inclusion bodies; Renaturation
REFOLD: An analytical database of protein refolding methods
Keywords: مجازات شامل; Inclusion bodies; Protein refolding; Renaturation; Database
Effect of temperature on protein quality in bacterial inclusion bodies
Keywords: مجازات شامل; Inclusion bodies; Protein aggregation; Protein folding; Recombinant protein expression; Escherichia coli
Structural analysis of protein inclusion bodies by Fourier transform infrared microspectroscopy
Keywords: مجازات شامل; Protein aggregation; Inclusion bodies; Heterologous protein production; FT-IR spectroscopy; Human growth hormone; Interferon alpha
Enhanced soluble protein expression using two new fusion tags
Keywords: مجازات شامل; Solubility tag; Protein expression; Inclusion bodies; Fusion proteins
Purification and antigenicity of two recombinant forms of Boophilus microplus yolk pro-cathepsin expressed in inclusion bodies
Keywords: مجازات شامل; Boophilus microplus; BYC; Thioredoxin; Inclusion bodies; N-Lauroyl sarcosine; Vaccine;
A new d-2-hydroxyacid dehydrogenase with dual coenzyme-specificity from Haloferax mediterranei, sequence analysis and heterologous overexpression
Keywords: مجازات شامل; D-2-hydroxyacid dehydrogenase; inclusion bodies; renaturalization; dual coenzyme-specificity; α-ketocarboxylic acid