An efficient procedure for the expression and purification of HIV-1 protease from inclusion bodies
Keywords: مجازات شامل; HIV-1 protease; Purification; pET32a(+); Inclusion bodies
مقالات ISI مجازات شامل (ترجمه نشده)
مقالات زیر هنوز به فارسی ترجمه نشده اند.
در صورتی که به ترجمه آماده هر یک از مقالات زیر نیاز داشته باشید، می توانید سفارش دهید تا مترجمان با تجربه این مجموعه در اسرع وقت آن را برای شما ترجمه نمایند.
در صورتی که به ترجمه آماده هر یک از مقالات زیر نیاز داشته باشید، می توانید سفارش دهید تا مترجمان با تجربه این مجموعه در اسرع وقت آن را برای شما ترجمه نمایند.
Expression and purification of biologically active recombinant human paraoxonase 1 from inclusion bodies of Escherichia coli
Keywords: مجازات شامل; Acyl homoserine lactone; Homocysteine thiolactone; In vitro refolding; Inclusion bodies; Organophosphate;
Efficient production of a correctly folded mouse α-defensin, cryptdin-4, by refolding during inclusion body solubilization
Keywords: مجازات شامل; α-Defensin; Coexpression; Inclusion bodies; Refolding; NMR
A purified truncated form of yeast Gal4 expressed in Escherichia coli and used to functionalize poly(lactic acid) nanoparticle surface is transcriptionally active in cellulo
Keywords: مجازات شامل; UAS-Gal4 system; Gal4; Escherichia coli; Inclusion bodies; PLA nanoparticle;
Efficient in vitro refolding and functional characterization of recombinant human liver carboxylesterase (CES1) expressed in E. coli
Keywords: مجازات شامل; Carboxylesterases; E. coli; Refolding; Inclusion bodies; Glycerol;
Characterization of recombinant pectate lyase refolded from inclusion bodies generated in E. coli BL21(DE3)
Keywords: مجازات شامل; Recombinant pectate lyase; Inclusion bodies; Refolding; CD spectra
High-yield reactivation of anionic tobacco peroxidase overexpressed in Escherichia coli
Keywords: مجازات شامل; Anionic tobacco peroxidase; Inclusion bodies; Recombinant protein expression; Escherichia coli; Protein refolding;
Engineering batch and pulse refolding with transition of aggregation kinetics: An investigation using green fluorescent protein (GFP)
Keywords: مجازات شامل; Inclusion bodies; Renaturation; Folding; Reaction order; GFP;
Mutations within A 35 amino acid region of P6 influence self-association, inclusion body formation, and Caulimovirus infectivity
Keywords: مجازات شامل; CaMV; TAV; Gene VI product; Inclusion bodies;
Improved Production and Characterization of Recombinant Human Granulocyte Colony Stimulating Factor from E. coli under Optimized Downstream Processes
Keywords: مجازات شامل; Fed-batch fermentation; Inclusion bodies; Reoxidation; Refolding kinetics; Cross flow filtration; Characterization;
One-step extraction of functional recombinant aquaporin Z from inclusion bodies with optimal detergent
Keywords: مجازات شامل; Aquaporin Z; Detergent; Protein purification; Solubility; Inclusion bodies;
High-level expression of angiotensin converting enzyme inhibitory peptide Tuna AI as tandem multimer in Escherichia coli BL21 (DE3)
Keywords: مجازات شامل; Bioactive peptide; Multimer; Fusion protein; Inclusion bodies; Purification; Antihypertensive activity;
Lethal Herpesvirosis in 16 Captive Horned Vipers (Vipera ammodytes ammodytes): Pathological and Ultrastructural Findings
Keywords: مجازات شامل; herpesvirus; inclusion bodies; transmission electron microscopy; Vipera ammodytes;
Role of the disaggregase ClpB in processing of proteins aggregated as inclusion bodies
Keywords: مجازات شامل; ClpB; Inclusion bodies; Molecular chaperone; Protein aggregation
Luminescence enhancement of the catalytic 19Â kDa protein (KAZ) of Oplophorus luciferase by three amino acid substitutions
Keywords: مجازات شامل; KAZ; the catalytic 19Â kDa protein of Oplophorus luciferase; nanoKAZ; a KAZ mutant substituted with 16 amino acid residues; eKAZ; a triple mutant of KAZ with V44I, A54I and Y138I; CTZ; coelenterazine; GLase; Gaussia luciferase; GLsp; the signal peptide se
A simple strategy for the purification of native recombinant full-length human RPL10 protein from inclusion bodies
Keywords: مجازات شامل; Inclusion bodies; Soluble protein; Binding zinc ions; Correct folding;
Expression and purification of mouse peptide ESP4 in Escherichia coli
Keywords: مجازات شامل; ESP; pET system; Escherichia coli expression; Inclusion bodies; Histidine-tag purification; Disulfide bridge;
Expression, purification and refolding of active durum wheat (Triticum durum Desf.) secretory phospholipase A2 from inclusion bodies of Escherichia coli
Keywords: مجازات شامل; Phospholipase A2; Recombinant protein; Escherichia coli; Inclusion bodies; Refolding;
Identification and active site analysis of the 1-aminocyclopropane-1-carboxylic acid oxidase catalysing the synthesis of ethylene in Agaricus bisporus
Keywords: مجازات شامل; ACC; 1-aminocyclopropane-1-carboxylic acid; ACO; ACC oxidase; Ab-ACO; Agaricus bisporus (J.E. Lange) Imbach ACC oxidase; SAM; S-adenosyl-L-methionine; ACS; ACC synthase; EFE; ethylene-forming enzyme; PCR; polymerase chain reaction; IPTG; β-D-thiogalactos
Mahogunin ring finger 1 suppresses misfolded polyglutamine aggregation and cytotoxicity
Keywords: مجازات شامل; AIP4; atrophin-interacting protein 4; Baf; bafilomycin; BMP; bis(monoacylglycero)phosphate; CMA; chaperone-mediated autophagy; CQ; chloroquine; HD; Huntington disease; HECT; homologous to E6-AP C-terminus; IB; inclusion bodies; Lamp2a; lysosome associated
Do-it-yourself histidine-tagged bovine enterokinase: A handy member of the protein engineer's toolbox
Keywords: مجازات شامل; BENAC; benzamidine affinity chromatography; EK; enterokinase; GST; glutathione-S-transferase; IB; inclusion body; IEC; ion exchange chromatography; IMAC; mmobilized metal ion affinity chromatography; KLK; kallikrein-related peptidase; SUMO; small ubiquiti
Employment of colorimetric enzyme assay for monitoring expression and solubility of GST fusion proteins targeted to inclusion bodies
Keywords: مجازات شامل; GST; GST-tagged proteins; Inclusion bodies; Urea;
The P6 protein of Cauliflower mosaic virus interacts with CHUP1, a plant protein which moves chloroplasts on actin microfilaments
Keywords: مجازات شامل; Intracellular movement; Subcellular localization; Inclusion bodies; CaMV;
Expression and purification of a novel therapeutic single-chain variable fragment antibody against BNP from inclusion bodies of Escherichia coli
Keywords: مجازات شامل; BNP; scFv Antibody; Inclusion bodies; Protein expression; Refolding
The effects of protein solubility on the RNA Integrity Number (RIN) for recombinant Escherichia coli
Keywords: مجازات شامل; Aggregation; Protease; Purification; Recombinant DNA; RNA; Inclusion bodies
Expression, refolding, and purification of active diacetylchitobiose deacetylase from Pyrococcus horikoshii
Keywords: مجازات شامل; Chitin; Diacetylchitobiose; Deacetylase; Inclusion bodies; Refolding;
Optimized refolding and characterization of active C-terminal ADAMTS-18 fragment from inclusion bodies of Escherichia coli
Keywords: مجازات شامل; rADAMTS-351; Inclusion bodies; Protein expression; Refolding; Platelet fragmentation;
Cooperation between two ClpB isoforms enhances the recovery of the recombinant β-galactosidase from inclusion bodies
Keywords: مجازات شامل; AAA+ ATPase; ClpB; Inclusion bodies; Molecular chaperone; Protein disaggregation; Protein reactivation
Recovery of active N-acetyl-d-glucosamine 2-epimerase from inclusion bodies by solubilization with non-denaturing buffers
Keywords: مجازات شامل; Epimerase; Inclusion bodies; Refolding; Solubilization
Integrated refolding techniques for Schistosoma japonicum MTH1 overexpressed as inclusion bodies in Escherichia coli
Keywords: مجازات شامل; Schistosoma japonicum; MutT homolog-1; Denaturation; Refolding; Inclusion bodies;
Efficient stable isotope labeling and purification of vitamin D receptor from inclusion bodies
Keywords: مجازات شامل; Vitamin D receptor; NMR (nuclear magnetic resonance); Isotope labeling; Inclusion bodies; Escherichia coli expression;
Solubilization of inclusion body proteins using n-propanol and its refolding into bioactive form
Keywords: مجازات شامل; Recombinant human growth hormone; Escherichia coli; Inclusion bodies; n-Propanol; Solubilization; Refolding and purification;
Expression, purification and analysis of an Arabidopsis recombinant CBL-interacting protein kinase3 (CIPK3) and its constitutively active form
Keywords: مجازات شامل; CIPK3; Site-directed mutagenesis; Inclusion bodies; Sarkosyl; Enzymatically active; Affinity purification; Protein kinase; Peptide phosphorylation
Substrate kringle-mediated catalysis by the streptokinase-plasmin activator complex: Critical contribution of kringle-4 revealed by the mutagenesis approaches
Keywords: مجازات شامل; Glu-PG; K1-K5CD (closed-conformer); Lys-PG; K1-K5CD (opened-conformer); K2-K5CD; K2K3K4K5CD; K3-K5CD; K3K4K5CD; K4K5CD; kringle 4 and kringle 5 conjugated with CD; K5CD; kringle 5 conjugated with CD; CD; catalytic domain of plasminogen; μPG; micr
Production of soluble recombinant proteins in Escherichia coli: Effects of process conditions and chaperone co-expression on cell growth and production of xylanase
Keywords: مجازات شامل; Escherichia coli; Inclusion bodies; Soluble expression; Molecular chaperones; Xylanase;
Centrosomal aggregates and Golgi fragmentation disrupt vesicular trafficking of DAT
Keywords: مجازات شامل; Aggresome; Centrosome; Dopamine transporter; γ-Tubulin; Golgi apparatus; Inclusion bodies; Lewy bodies; Microtubules; Neurodegeneration; Parkinson's disease; Proteasome; Protein aggregation;
Autophagy and polyglutamine diseases
Keywords: مجازات شامل; HD; Huntington's disease; SCA; spinocerebellar ataxia; DRPLA; Denatorubral-pallidoluysian atrophy; SBMA; spinal and bulbar muscular atropy; Htt; Huntingtin; UPS; ubiquitin-proteasome system; HDL-2; Huntington's disease-like 2; IBs; inclusion bodies; RNA
The Dahlia mosaic virus gene VI product N-terminal region is involved in self-association
Keywords: مجازات شامل; Caulimovirus; DMV; DNA virus; Inclusion bodies; MBP pull-down; TAV; Yeast two-hybrid
FhuA deletion variant Δ1-159 overexpression in inclusion bodies and refolding with Polyethylene-Poly(ethylene glycol) diblock copolymer
Keywords: مجازات شامل; Channel proteins; FhuA; Diblock copolymer; Inclusion bodies; Protein purification; β-barrel proteins
On-column refolding purification of DT389-hIL13 recombinant protein expressed in Escherichia coli
Keywords: مجازات شامل; Inclusion bodies; On-column refolding; Size exclusion chromatography; Urea gradient; pH gradient
Expression, purification, and refolding of biologically active Acinetobacter baumannii OmpA from Escherichia coli inclusion bodies
Keywords: مجازات شامل; Acinetobacter baumannii; OmpA; Inclusion bodies; Refolding
A strategy for high-level expression of a single-chain variable fragment against TNFα by subcloning antibody variable regions from the phage display vector pCANTAB 5E into pBV220
Keywords: مجازات شامل; TNFα; scFv; Phage display; Inclusion bodies; E. coli
K-law spectral signature correlation algorithm to identify white spot syndrome virus in shrimp tissues
Keywords: مجازات شامل; White spot syndrome virus; Inclusion bodies; Digitalized images; Spectral signature
Binding specificity of the ectodomain of the parathyroid hormone receptor
Keywords: مجازات شامل; Ibs; inclusion bodies; IMAC; immobilized metal ion affinity chromatography; PTH1R; parathyroid hormone receptor; GPCR; G protein-coupled receptor; Parathyroid hormone (PTH); Parathyroid hormone receptor 1 (PTH1R); GPCR; Peptide spotting; Isothermal titrat
Expression, refolding and purification of a human interleukin-17A variant
Keywords: مجازات شامل; Interleukin-17A; Inclusion bodies; Refolding; Chromatography;
Expression of soluble proteins in Escherichia coli by linkage with the acidic propiece of eosinophil major basic protein
Keywords: مجازات شامل; Inclusion bodies; C5a; CCL18; FGFβ; LIF; Protein expression; Recombinant cationic proteins; Solubility enhancement; Complement; Stem cells
A novel protein refolding system using lauroyl-l-glutamate as a solubilizing detergent and arginine as a folding assisting agent
Keywords: مجازات شامل; Protein refolding; Inclusion bodies; Solubilization; C12-l-Glu; Arginine;
A high-throughput protein refolding screen in 96-well format combined with design of experiments to optimize the refolding conditions
Keywords: مجازات شامل; Design of experiments (DOE); Inclusion bodies; Refolding screen; IL-17F; SDF-1α; BCA-1; GM-CSF; C5a;
Purification and refolding of recombinant human interferon-gamma in urea–ammonium chloride solution
Keywords: مجازات شامل; Human interferon-gamma; hIFNγ; hIFNγ purification; Inclusion bodies; Recombinant interferon-gamma
Escherichia coli expression, refolding and characterization of human laforin
Keywords: مجازات شامل; Laforin; Lafora disease; Inclusion bodies; Refolding; Dual-specificity phosphatase; Carbohydrate binding module