Expression, refolding, and purification of active diacetylchitobiose deacetylase from Pyrococcus horikoshii

► The deacetylase from Pyrococcus horikoshii (Ph-Dac) was overexpressed as inclusion bodies in Escherichia coli. ► Ph-Dac was refolded as the bioactive homohexamer form. ► Ph-Dac exhibited specific deacetylase activity toward (GlcNAc)2.