Keywords: بازپرداخت; Inclusion body; Chemical solubilization; Refolding; Biological potency; Secondary structure; Circular dichroism;
مقالات ISI بازپرداخت (ترجمه نشده)
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Keywords: بازپرداخت; Prion; Biological safety level; Protein; Expression; Purification; Refolding; PrPC; major prion protein of higher animals (cellular form); PrPSC; major prion protein of higher animals (scrapie form); SDS-PAGE; sodium dodecyl sulfate polyacrylamide gel ele
Keywords: بازپرداخت; Recombinant human bone morphogenetic protein-4 (rhBMP-4); Inclusion bodies; Refolding; Cation-exchange membrane chromatography;
Keywords: بازپرداخت; HSA; serum albumin; G5; pentanediyl-α,Ï-bis(cetyldimethylammonium bromide); CD; circular dichroism; DLS; dynamic light scattering; Refolding; Aggregation; Denaturation; Artificial chaperone; HSA; GdCl; Single-chain surfactant; CTAB; G5; Gemini surfactan
Keywords: بازپرداخت; Refolding; Outer membrane proteins; AlkL; Detergents; Inclusion bodies;
Keywords: بازپرداخت; PEDV S protein; Inclusion bodies; Solubilization; Refolding; Immunogenicity; Subunit vaccinePEDV, porcine epidemic diarrhea virus; IBs, inclusion bodies; IPTG, isopropyl d-1-thiogalactopyranoside; OD, optical density; DTT, dithiothreitol; TBS, Tris-buffer
Keywords: بازپرداخت; Photoprotein; Thermodynamic stability; Refolding; Unfolding; Kinetics; Intermediate;
Keywords: بازپرداخت; Histone octamer; Nucleosome; Refolding; Histone methyltransferase; Small angle X-ray scattering
Keywords: بازپرداخت; Catalytic activity; Chemiluminescence; Recombinant tobacco peroxidase; Refolding; Site-directed mutagenesis;
Keywords: بازپرداخت; Refolding; High hydrostatic pressure; Enhanced green fluorescent protein; Temperature; Inclusion body
Keywords: بازپرداخت; protein aggregates; monoclonal antibody; oligomer; disaggregation; refolding; thermal-cycling; chromatography; protein folding/refolding; stability; circular dichroism;
Keywords: بازپرداخت; Refolding; Ultrafiltration; rhG-CSF; Inclusion bodies; Polyethylene glycol; Dextran;
Keywords: بازپرداخت; High hydrostatic pressure; Refolding; Endostatin; Inclusion bodies; Aggregates;
Keywords: بازپرداخت; Albumin protein; Unfolding; Refolding; Binding; Structure and function;
Surface plasmon resonance (SPR) based binding studies of refolded single chain antibody fragments
Keywords: بازپرداخت; Immunoglobulins; Recombinant antibody; Bacterial expression system; Affinity purification; Refolding; SPR;
Effects of osmolytes on the refolding of recombinant Pelodiscus sinensis brain-type creatine kinase
Keywords: بازپرداخت; P-CKB; brain type of creatine kinase from Pelodiscus sinensis; ANS; 1-anilinonaphthalene-8-sulfonate; ATP; adenosine triphosphate; SDS-PAGE; sodium dodecyl sulfate-polyacrylamide gel electrophoresis; Pelodiscus sinensis; Creatine kinase; Urea; Osmolytes;
Leptospira interrogans thermolysin refolded at high pressure and alkaline pH displays proteolytic activity against complement C3
Keywords: بازپرداخت; GdnHCl; guanidine hydrochloride; HHP; high hydrostatic pressure; IPTG; isopropyl β-D-thiogalactopyranoside; LB; Luria-Bertani broth; LS; light scattering; TL-IB; inclusion bodies of thermolysin; SDS-PAGE; sodium dodecyl sulphate polyacrylamide gel electr
Cloning and characterization of serpin from red king crab Paralithodes camtschaticus
Keywords: بازپرداخت; Serpin; Crab; Paralithodes camtschaticus; Immunity; Phenoloxidase; Inhibitor; Recombinant; Refolding;
Molecular cloning, prokaryotic expression, purification, structural studies and functional implications of Heat Shock Protein 70 (Hsp70) from Rutilus frisii kutum
Keywords: بازپرداخت; Hsp70; Rutilus frisii kutum; ATP hydrolysis; Luciferase; Aggregation; Refolding;
Properties of dimeric, disulfide-linked rhBMP-2 recovered from E. coli derived inclusion bodies by mild extraction or chaotropic solubilization and subsequent refolding
Keywords: بازپرداخت; BRE-Luc assay; Cystine-knot; Disulfide-bond; Heparin-affinity chromatography; Mild extraction; Purification; Refolding; Recombinant human bone morphogenetic protein-2;
Optimization of in vitro refolding conditions of recombinant Lepidium draba peroxidase using design of experiments
Keywords: بازپرداخت; Design of experiments; Lepidium draba peroxidase; Refolding; Response surface methodology;
Folding and stabilizing membrane proteins in amphipol A8-35
Keywords: بازپرداخت; 3D; three-dimensional; A8-35; a polyacrylate-based amphipol; APol; amphipol; BO; bacterio-opsin; BR; bacteriorhodopsin from Halobacterium salinarum; Ca2+-ATPase; the sarcoplasmic calcium pump; cac; critical aggregation concentration; CD; circular dichrois
Efficient renaturation of inclusion body proteins denatured by SDS
Keywords: بازپرداخت; Inclusion bodies; Refolding; SDS; Ulvan lyase; SDS; sodium dodecyl sulfate; IPTG; isopropyl β-d-1-thiogalactopyranoside;
Refolding of a novel cholesterol oxidase from Pimelobacter simplex reveals dehydrogenation activity
Keywords: بازپرداخت; Cholesterol oxidase; Dehydrogenase; Refolding; Enzymatic activity; Cholest-5-en-3-one; Biocatalyst;
High hydrostatic pressure enables almost 100% refolding of recombinant human ciliary neurotrophic factor from inclusion bodies at high concentration
Keywords: بازپرداخت; rhCNTF; High hydrostatic pressure; Inclusion bodies; Hydrophobic aggregation; Refolding;
High-level expression and efficient refolding of therapeutically important recombinant human Interleukin-3 (hIL-3) in E. coli
Keywords: بازپرداخت; Escherichia coli; Human interleukin-3 (hIL-3); Codon-optimization; Circular dichroism; Refolding; TF-1Â cells;
A comparative approach to recombinantly produce the plant enzyme horseradish peroxidase in Escherichia coli
Keywords: بازپرداخت; Horseradish peroxidase; Escherichia coli; Periplasm; Inclusion body; Refolding; ABTS; 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid); Amp; Ampicillin; CV; column volume; DCW; dry cell weight; DSP; downstream processing; DTT; Dithiothreitol; H2O
Prevention of aggregate formation through mechanism analysis in refolding of recombinant pertactin from Escherichia coli
Keywords: بازپرداخت; Recombinant pertactin; Refolding; Aggregates; Dilution;
Co-refolding of a functional complex of Dengue NS3 protease and NS2B co-factor domain and backbone resonance assignment by solution NMR
Keywords: بازپرداخت; Dengue virus; NS3 protease; Flavivirus protease; Refolding; Protein purification; Resonance assignment; Protein complex;
Ubiquitin phosphorylated at Ser57 hyper-activates parkin
Keywords: بازپرداخت; E3 ligase; Genetic code expansion; Parkinson's disease; Post-translational modification; Refolding; SepRS; tRNASep; Ubiquitin;
Characterization of the sensor domain of QseE histidine kinase from Escherichia coli
Keywords: بازپرداخت; Quorum sensing; Histidine kinase; QseE; QseE sensor domain; Refolding;
Expression, purification and characterization of a full-length recombinant HIV-1 Vpu from inclusion bodies
Keywords: بازپرداخت; HIV-1; Vpu; Over-expression; Solubilization; Purification; Refolding; ER; endoplasmic reticulum; βTrCP; beta-transducine repeat containing protein; BST-2; bone marrow stromal antigen 2; IPTG; Isopropyl β-D-1-thiogalactopyranoside; DTT; Dithiothreitol; P
Efficient refolding of the bifunctional therapeutic fusion protein VAS-TRAIL by a triple agent solution
Keywords: بازپرداخت; VAS-TRAIL fusion protein; Triple agent solution; Mild solubilization; l-Arginine; Refolding
Expression, purification, refolding and in vitro recovery of active full length recombinant human gelatinase MMP-9 in Escherichia coli
Keywords: بازپرداخت; Matrix metalloproteinases (MMPs); Active full length recombinant human gelatinase MMP-9 (rh MMP-9); Inclusion body; Refolding;
Insights into the structure-function relationship of Brugia malayi thymidylate kinase (BmTMK)
Keywords: بازپرداخت; Brugia malayi; Thymidylate kinase; Drug target; Unfolding; Refolding; Inhibition
Heterologous expression and pro-peptide supported refolding of the high specific endopeptidase Lys-C
Keywords: بازپرداخت; Lysyl endopeptidase; Lysobacter enzymogenes; Pro-peptide; Refolding; Cultivation; Escherichia coli
Recombinant expression, refolding, purification and characterization of Pseudomonas aeruginosa protease IV in Escherichia coli
Keywords: بازپرداخت; Pseudomonas aeruginosa; Protease IV; Recombinant expression; Refolding; Purification; Activity; TRX; thioredoxin; LB medium; Luria-Bertani medium; ORF; open reading frame; DO; dissolved oxygen; FCM; fermentation complex medium; IPTG; isopropyl thio-β-d-g
Production strategies for active heme-containing peroxidases from E. coli inclusion bodies – a review
Keywords: بازپرداخت; E. coli; Peroxidase; Inclusion body; Solubilization; Refolding
Nano-cage-mediated refolding of insulin by PEG-PE micelle
Keywords: بازپرداخت; Insulin; DTT; Refolding; PEG-PE micelle; Nano-cage; Negative charge layer; DTT; dithiothreitol; CD; circular dichroism; ThT; thioflavinT; HSPs; heat-shock proteins; apoE; apolipoprotein E; SOD1; superoxide dismutase 1; IAPP; islet amyloid polypeptide; PDI
Refolding, purification, and preliminary structural characterization of the DNA-binding domain of the quorum sensing receptor RhlR from Pseudomonas aeruginosa
Keywords: بازپرداخت; RhlR; Quorum sensing; Receptor; Refolding; Purification
High-level expression of pseudolysin, the extracellular elastase of Pseudomonas aeruginosa, in Escherichia coli and its purification
Keywords: بازپرداخت; Pseudolysin; Elastase; Over-expression; Purification; Precipitation; Refolding;
Investigation on solubilization protocols in the refolding of the thioredoxin TsnC from Xylella fastidiosa by high hydrostatic pressure approach
Keywords: بازپرداخت; High hydrostatic pressure; Thioredoxin; TsnC; Refolding;
Transient conformational modification of immunoglobulin G during purification by protein A affinity chromatography
Keywords: بازپرداخت; Protein A; IgG purification; IgG size; Denaturation; Refolding; Arginine;
Expression, purification, refolding, and enzymatic characterization of two secretory phospholipases A2 from Neurospora crassa
Keywords: بازپرداخت; CD; circular dichroism; ESI-MS; electrospray ionization mass spectrometry; His6; hexahistidine; LPC; lysophosphatidylcholine; LPE; lysophosphatidylethanolamine; NDSB; non-detergent sulfobetain; ORF; open reading frame; PC; phosphatidylcholine; PE; phospha
Expression, refolding, and characterization of a small laccase from Thermus thermophilus HJ6
Keywords: بازپرداخت; Laccase; Refolding; Thermus thermophilus; Thermostability;
Efficient in vitro refolding and functional characterization of recombinant human liver carboxylesterase (CES1) expressed in E. coli
Keywords: بازپرداخت; Carboxylesterases; E. coli; Refolding; Inclusion bodies; Glycerol;
Efficient production of a correctly folded mouse α-defensin, cryptdin-4, by refolding during inclusion body solubilization
Keywords: بازپرداخت; α-Defensin; Coexpression; Inclusion bodies; Refolding; NMR
Characterization of recombinant pectate lyase refolded from inclusion bodies generated in E. coli BL21(DE3)
Keywords: بازپرداخت; Recombinant pectate lyase; Inclusion bodies; Refolding; CD spectra
The zymogen of plasmepsin V from Plasmodium falciparum is enzymatically active
Keywords: بازپرداخت; DABCYL; 4-((4-(dimethylamino)phenyl)azo)benzoic acid; EDANS; 5-((2-aminoethyl)amino)naphthalene-1-sulfonic acid; KNI; kinostatin; CHAPS; 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate; CHES; 2-(cyclohexylamino)ethanesulfonic acid; MES; 2-(N-mor
The role of interchain disulfide bond in a recombinant human interleukin-17A variant
Keywords: بازپرداخت; CD; Circular Dichroism; CHCA; alpha-cyano-4-hydroxycinnamic acid; DSC; differential scanning calorimetry; HPBA; heptafluoro-butyric anhydride; MMtheo; theoretical molar mass; MMmeas; measured molar mass; PrOH; propanol; IL-17A; Refolding; Disulfide bond;