کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8360000 1542326 2015 21 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
One-step extraction of functional recombinant aquaporin Z from inclusion bodies with optimal detergent
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
One-step extraction of functional recombinant aquaporin Z from inclusion bodies with optimal detergent
چکیده انگلیسی
Aquaporins are integral membrane channel proteins found in all kingdoms of life. The Escherichia coli aquaporin Z (AqpZ) has been shown to solely conduct water at high permeability. Functional AqpZ is generally purified from the membrane fraction. However, the quantity of the purified protein is limited. In this study, a new method is developed to achieve high yield of bioactive AqpZ protein. A mild detergent n-dodecyl-β-d-maltopyranoside (DDM) was used to solubilize the over-expressed insoluble AqpZ from inclusion bodies without a refolding process. The recovered AqpZ protein showed high water permeability comparable with AqpZ obtained from the membrane fraction. In this way, the total yield of bioactive AqpZ has been increased greatly, which will facilitate the structural and functional characterization and future applications of AqpZ.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 115, November 2015, Pages 146-152
نویسندگان
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