Keywords: PI، نقطه ایزوالکتریک; NMR, naked mole rat; SAAC, single amino acid change; PPI, protein–protein interaction; pI, isoelectric point; FDR, false discovery rate; AGCOH, Atlas of Genetics and Cytogenetics in Oncology and Haematology; OMIM, Online Mendelian Inheritance in ManCancer
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Identification, structural characterisation and expression analysis of a defensin gene from the tiger beetle Calomera littoralis (Coleoptera: Cicindelidae)
Keywords: PI، نقطه ایزوالکتریک; AMP, antimicrobial peptides; bp, base pair; cDNA, complementary DNA; Cys, cystein; Mw, molecular weight; pI, isoelectric point; RACE, rapid amplification of cDNA ends; RT-qPCR, real time quantitative PCRAMP; Adephagan beetles; Cicindelids; Defensin; RT-qP
Expression of stem cell pluripotency factors during regeneration in the earthworm Eisenia foetida
Keywords: PI، نقطه ایزوالکتریک; RACE, rapid amplification of cDNA ends; PCR, polymerase chain reaction; NCBI, National Center of Biotechnology Information; ORF, open reading frame; pI, isoelectric point; MW, molecular weight; BLAST, Basic Local Alignment Search Tool; NJ, neighborjoining
Protein abundance changes of Zygosaccharomyces rouxii in different sugar concentrations
Keywords: PI، نقطه ایزوالکتریک; G6P, Glucose-6-phosphate; X5P, D-xylulose 5-phosphate; G3P, Glycerol-3-phosphate; 1,3BP, 1,3 Bisphosphoglycerate; S7P, Sedoheptulose 7-phosphate; RL5P, D-ribulose 5-phosphate; pI, isoelectric point; no., number; F6P, Fructose 1,6-bishosphate; E4P, D-eryth
Genome-wide analysis of WRKY family of transcription factors in common bean, Phaseolus vulgaris: Chromosomal localization, structure, evolution and expression divergence
Keywords: PI، نقطه ایزوالکتریک; TFs, transcription factors; RNA-seq, RNA sequencing; HMM, hidden Markov model; MW, molecular weight; pI, isoelectric point; aa, amino acid(s); AtWRKY, WRKY transcription factors in Arabidopsis; PvWRKYs, WRKY transcription factors in Phaseolus vulgaris; P.
Genome-wide identification, classification and expression analysis of the PHD-finger protein family in Populus trichocarpa
Keywords: PI، نقطه ایزوالکتریک; PHD, plant homeobox domain; pI, isoelectric point; Mya, million years ago; CDS, coding sequences; HMM, hidden Markov model; NJ, neighbor-joining; At, Arabidopsis thaliana; Pt, Populus trichocarpa; GEO, Gene Expression Omnibus; NCBI, National Center for Bi
Genome-wide identification and comparative expression analysis reveal a rapid expansion and functional divergence of duplicated genes in the WRKY gene family of cabbage, Brassica oleracea var. capitata
Keywords: PI، نقطه ایزوالکتریک; TFs, transcription factors; WGT, whole-genome triplication; RNA-seq, RNA sequencing; NCBI, National Center for Biotechnology Information; SRA, Sequence Read Archive; HMM, hidden Markov model; MW, molecular weight; pI, isoelectric point; aa, amino acid(s);
Comprehensive genomic analysis and expression profiling of diacylglycerol kinase gene family in Malus prunifolia (Willd.) Borkh
Keywords: PI، نقطه ایزوالکتریک; ABA, abscisic acid; SA, salicylic acid; GA, gibberellins; MeJA, methyl jasmonate; DGK, diacylglycerol kinase; PI, isoelectric point; qRT-PCR, quantitative real-time PCRApple; Diacylglycerol kinase; Gene family; Abiotic stress
Hepatic proteomic analysis revealed altered metabolic pathways in insulin resistant Akt1+/−/Akt2−/− mice
Keywords: PI، نقطه ایزوالکتریک; IRS, insulin receptor substrate; PI3K, phosphoinositide 3-kinase; 2-D DIGE, two-dimensional differential gel electrophoresis; pI, isoelectric point; MALDI, matrix-assisted laser desorption/ionization; TOF, time of flightMouse; Liver; AKT; Insulin resistan
The study of fkbp and ubiquitin reveals interesting aspects of Artemia stress history
Keywords: PI، نقطه ایزوالکتریک; FKBP, FK506-Binding Protein; HSPs, heat shock proteins; PIKKs, phosphoinositide 3-kinase related kinases; PPIase, peptidyl prolyl cis/trans isomerase; TPRs, tetratricopeptide repeats; MM, molecular mass; pI, isoelectric point; GRAVY, grand average of hydr
Use of mep HyperCel for polishing of human serum albumin
Keywords: PI، نقطه ایزوالکتریک; ID, Internal diameter; ELISA, Enzyme linked immunosorbent assay; DEAE, Diethylaminoethyl; CM, Carboxymethyl; pI, Isoelectric point; HCIC, Hydrophobic charge induction chromatographyMEP HyperCel; Human serum albumin; Hydrophobic charge induction chromatogr
HPLC separation of human serum albumin isoforms based on their isoelectric points
Keywords: PI، نقطه ایزوالکتریک; EPGC, external pH gradient chromatofocusing; AEHIC, anion exchange-hydrophobic interaction chromatography; DTNB, 5,5′-dithiobis-(2-nitrobenzoate); DEAE, diethylaminoethyl; pI, isoelectric point; GSH, reduced glutathione; NAC, N-acetylcysteine; HSA, human
Cloning, expression and cellular localization of the Doublesex gene in the water flea, Daphnia carinata, during different developmental stages
Keywords: PI، نقطه ایزوالکتریک; RACE, rapid amplification of cDNA ends; Dsx, Doublesex; DapcaDsx1, Daphnia carinata Doublesex 1; PCR, Polymerase chain reaction; PFA, paraformaldehyde; NCBI, National Center of Biotechnology Information; ORF, open reading frame; pI, isoelectric point; MW,
cIEF for rapid pKa determination of small molecules: A proof of concept
Keywords: PI، نقطه ایزوالکتریک; ADME, absorption, distribution, metabolism and excretion; CA, carrier ampholytes; CE, capillary electrophoresis; cIEF, capillary isoelectric focusing; EOF, electroosmotic flow; FS, fused silica; HPC, hydroxypropylcellulose; pI, isoelectric point; pKa, ion
Identification of alternatively spliced isoforms of interleukin-2/15 receptor β chain in ducks
Keywords: PI، نقطه ایزوالکتریک; A region, acidic-rich region; bp, base pair; FN3, fibronectin type 3; γc, gamma chain; Jak, Janus tyrosine kinase; kb, kilobase; kDa, kilodalton; ORF, open reading frame; PBS-T, PBS with 0.1% Tween 20; pI, isoelectric point; PIP, percent identity plot; qR
Expression profiling of the triterpene saponin biosynthesis genes FPS, SS, SE, and DS in the medicinal plant Panax notoginseng
Keywords: PI، نقطه ایزوالکتریک; MeJA, methyl jasmonate; Ct, cycle threshold; ORF, open reading frame; EST, expressed sequence tag; NCBI, National Center for Biotechnology Information; kDa, kilo Dalton; pI, isoelectric point; MVA, mevalonic acid; FPP, farnesyl diphosphate; FPS, farnesyl
Proteomics of old world camelid (Camelus dromedarius): Better understanding the interplay between homeostasis and desert environment
Keywords: PI، نقطه ایزوالکتریک; 2D, two-dimensional; MS, mass spectrometry; CHAPS, 3-(3-cholamidopropyl)-dimethylammoniopropane sulfonate; pI, isoelectric point; IPG, immobilized pH gradient; DTT, dithiothreitol; SDS, sodium dodecylsulfate; PAGE, polyacrylamide gel electrophoresis; TFA,
A proteomics approach to detect tissue-wide adaptive changes in the pancreas associated with increased pancreatic α-amylase activity in domestic cattle (Bos taurus)
Keywords: PI، نقطه ایزوالکتریک; 2D-HPLC, two-dimensional high-performance liquid chromatography; MS, mass spectrometry; MS/MS, tandem mass spectrometry; pI, isoelectric point; NanoES Q-TOF MS, nano-electrospray quadrupole time-of-flight mass spectrometry; MOWSE score, Molecular Weight S
Kinetic and thermodynamic analysis of the inhibitory effects of maltose, glucose, and related carbohydrates on wheat β-amylase
Keywords: PI، نقطه ایزوالکتریک; BacBA, Bacillus β-amylase; BBA, barley β-amylase; EI, enzyme–inhibitor complex; pI, isoelectric point; SBA, soybean β-amylase; WBA, wheat β-amylaseGlucose; Inhibition; Maltose; Product inhibition; Wheat β-amylase
Characterization and functional cloning of an aromatic nitrilase from Pseudomonas putida CGMCC3830 with high conversion efficiency toward cyanopyridine
Keywords: PI، نقطه ایزوالکتریک; DTT, dithiothreitol; LB, Luria–Bertani; pI, isoelectric point; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; HPLC, high performance liquid chromatography; Vmax, maximal hydrolysis rate; Km, apparent Michaelis–Menten constant; ORF, o
Molecular cloning and catalytic characterization of a recombinant tropine biosynthetic tropinone reductase from Withania coagulans leaf
Keywords: PI، نقطه ایزوالکتریک; WcTR-I, Withania coagulans tropinone reductase I; PCR, Polymerase chain reaction; RACE, Rapid amplification of cDNA ends; ORF, Open reading frame; kDa, kilo Dalton; pI, Isoelectric point; NADPH, β-nicotinamide adenine dinucleotide phosphate reduced; SDRs,
Isolation and characterization of terpene synthases potentially involved in flavor development of ripening olive (Olea europaea) fruits
Keywords: PI، نقطه ایزوالکتریک; cv., cultivar; DAF, days after flowering; EST, expressed sequence tag; ORF, open reading frame; PCR, polymerase chain reaction; pI, isoelectric point; ppm, parts per million; RACE, rapid amplification of cDNA ends; SI, sequence identity; SPME, solid phase
IVIg-mediated inhibition of antigen presentation: Predominant role of naturally occurring cationic IgG
Keywords: PI، نقطه ایزوالکتریک; IVIg, intravenous immunoglobulins; cIVIg, cationized IVIg; OVA, ovalbumin; HSA, human serum albumin; cHSA, cationized HSA; pI, isoelectric point; MFI, mean fluorescence intensityIVIg; IgG isoelectric point; APC; Internalization; Antigen presentation; T ce
Protein quantitation using various modes of high performance liquid chromatography
Keywords: PI، نقطه ایزوالکتریک; API, active pharmaceutical ingredient; CME, cellulose mixed ester; mAb, monoclonal antibody; nSEC, Non Ideal size exclusion chromatography; pI, isoelectric point; SAX, strong anion exchange; TFA, trifluoroacetic acid; tR, retention time; WCX, weak cation
Proteomic study of β-aminobutyric acid-mediated cadmium stress alleviation in soybean
Keywords: PI، نقطه ایزوالکتریک; 2-DE, two-dimensional polyacrylamide gel electrophoresis; pI, isoelectric point; BABA, β-aminobutyric acid; PC, phytochelatin; GS, glutamine synthetaseCadmium; Soybean; Proteomics; β-Aminobutyric acid; Priming; Biophoton
Organ-specific proteomic analysis of drought-stressed soybean seedlings
Keywords: PI، نقطه ایزوالکتریک; 2-DE, two-dimensional polyacrylamide gel electrophoresis; CBB, Coomassie brilliant blue; MS, mass spectrometry; LC, liquid column; pI, isoelectric point; IEF, isoelectric focusing; PEG, polyethylene glycol; qRT-PCR, quantitative reverse-transcription real
G-protein coupled receptor-associated sorting protein 1 (GASP-1), a potential biomarker in breast cancer
Keywords: PI، نقطه ایزوالکتریک; GASP-1, G-protein coupled receptor-associated sorting protein 1; 2-D HPLE, 2-D high performance liquid electrophoresis; PBS, phosphate buffered saline; PAGE, polyacrylamide gel electrophoresis; PVDF, polyvinylidene fluoride; PI, isoelectric point; HRP, ho
Anionic and cationic dextran hydrogels for post-loading and release of proteins
Keywords: PI، نقطه ایزوالکتریک; Dex-MA, methacrylated dextran; MA, methacrylic acid; DMAEMA, 2-N,N-dimethylaminoethyl methacrylate; HEMA, hydroxyethyl methacrylate; HBS, HEPES buffered saline; BSA, bovine serum albumin; pI, isoelectric point; TEMED, N,N,N′,N′-tetramethylethylenediamine;
Foaming properties of tryptic gliadin hydrolysate peptide fractions
Keywords: PI، نقطه ایزوالکتریک; AA, amino acid(s); CD, central domain(s); db, on dry basis; DH, degree of hydrolysis (%); FS, foam stability; FV, initial foam volume; MW, molecular weight; PES, polyethersulfone; pI, isoelectric point; RP-HPLC, reversed-phase HPLC; SE-HPLC, size-exclusio
Expression of three galactinol synthase isoforms in Coffea arabica L. and accumulation of raffinose and stachyose in response to abiotic stresses
Keywords: PI، نقطه ایزوالکتریک; Coffee; Water deficit; Heat stress; Salinity; Galactinol synthase; Raffinose family oligosaccharidesDW, dry weight; GolS, galactinol synthase; HSFs, heat shock transcription factors; pI, isoelectric point; RFOs, raffinose family oligosaccharides; ROS, rea
Enhanced pulmonary absorption of a macromolecule through coupling to a sequence-specific phage display-derived peptide
Keywords: PI، نقطه ایزوالکتریک; HEPES, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; OG, Oregon Green 488®; PBS, phosphate buffered saline pH 7.4; I.P., intra-peritoneal; pMDI, pressurised metered dose inhaler; GPC, gel permeation chromatography; pI, isoelectric point; MTT, 3-[4,5
Pyroglutamate-Aβ: Role in the natural history of Alzheimer's disease
Keywords: PI، نقطه ایزوالکتریک; Aβ, amyloid-beta; APP, amyloid precursor protein; BACE, β-site APP cleaving enzyme; pE, pyroglutamate; pI, isoelectric point; ROS, reactive oxygen species; QC, glutaminyl cyclasePyroglutamate; Amyloid-beta; Alzheimer's disease; Glutaminyl cyclase
Comparative protein profiles: Potential molecular markers from spermatozoa of Acipenseriformes (Chondrostei, Pisces)
Keywords: PI، نقطه ایزوالکتریک; MALDI-TOF/MS, matrix-associated laser desorption/ionization time-of-flight/mass spectrometry; 2DE, two-dimensional polyacrylamide gel electrophoresis; IPG, immobilized pH gradient; MeCN, acetonitrile; pI, isoelectric point; MM, molecular mass; PMF, peptid
Fruit-specific expression of papaya subtilase gene
Keywords: PI، نقطه ایزوالکتریک; cDNA clone; Expression; Genomic DNA; Serine protease; SubtilaseHRP, horseradish peroxidase; IgG, immunoglobulin G; IPTG, isopropylthio-β-d-galactoside; LB, Luria Bertani; ORF, open reading frame; PCR, polymerase chain reaction; pI, isoelectric point; RACE
Proteomic analysis of serum of workers occupationally exposed to arsenic, cadmium, and lead for biomarker research: A preliminary study
Keywords: PI، نقطه ایزوالکتریک; PCA, Principle Component Analysis; IEF, isoelectric focusing; 2-DE, two-dimensional gel electrophoresis; pI, isoelectric point; Mw, molecular weightBiomarkers; Proteomic profiles; Metal mixture exposure; Two-dimensional gel electrophoresis; Mathematical a
Comparative study of anticoagulant and procoagulant properties of 28 snake venoms from families Elapidae, Viperidae, and purified Russell’s viper venom-factor X activator (RVV-X)
Keywords: PI، نقطه ایزوالکتریک; Russell’s viper venom-factor X activator; Activated clotting time; Clot rate; Procoagulant; AnticoagulantACT, activated clotting time; ET, electrophoretic titration; pI, isoelectric point; RVV, Russell’s viper venom; RVV-X, Russell’s viper venom-factor X
Evolution of ASABF (Ascaris suum antibacterial factor)-type antimicrobial peptides in nematodes: Putative rearrangement of disulfide bonding patterns
Keywords: PI، نقطه ایزوالکتریک; ASABF, Ascaris suum antibacterial factor; pI, isoelectric point; EST, expressed sequence tag; RACE, rapid amplification of cDNA ends; RT, reverse transcription; EF1α, elongation factor 1α; SL1, nematode splice leader sequence 1Defensin; CSαβ; Phylogeny; I
A proteomic reference map for pig serum proteins as a prerequisite for diagnostic applications
Keywords: PI، نقطه ایزوالکتریک; 1D, one-dimensional; 2-DE, two-dimensional electrophoresis; App, Actinobacillus pleuropneumoniae; Ig, immunoglobulin; IPG, immobilized pH-gradient; MAP, major acute phase protein; PCV2, porcine circovirus type 2; pI, isoelectric point; PMWS, postweaning m
Estimation and characterisation of major royal jelly proteins obtained from the honeybee Apis merifera
Keywords: PI، نقطه ایزوالکتریک; RJ, royal jelly; MRJP, major royal jelly protein; SDS–PAGE, sodium dodecyl sulfate-polyacylamidogelelectrophoresis; 2-DE, 2-dimensional electrophoresis; pI, isoelectric point; IEF, isoelectric focusing; MALDI TOF/TOF MS, matrix assisted laser desorption i
Physicochemical and functional properties of acidic and basic polypeptides of soy glycinin
Keywords: PI، نقطه ایزوالکتریک; pI, isoelectric point; 2-ME, β-mercaptoethanol; SHT, total free sulphydryl; SHE, exposed free sulphydryl; SDS–PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; DTNB, 5,5′-dinitro-bis-(2-nitrobenzoic acid); EDTA, ethylenediamine tetraacetic
Molecular cloning and characterization of a thaumatin-like protein-encoding cDNA from rough lemon
Keywords: PI، نقطه ایزوالکتریک; Citrus jambhiri; Thaumatin-like protein; Anti-fungal activity; Alternaria alternataGFP, green fluorescent protein; pI, isoelectric point; IPTG, isopropyl-1-thio-beta-galactoside; TLP, thaumatin-like protein; RACE, rapid amplification of cDNA ends; PCR, po
Proteomics approach for identifying osmotic-stress-related proteins in soybean roots
Keywords: PI، نقطه ایزوالکتریک; 2-DE, two-dimensional polyacrylamide gel electrophoresis; CBB, Coomassie brilliant blue; IEF, isoelectric focusing; pI, isoelectric point; Mr, relative molecular mass; MALDI-TOF MS, matrix-assisted laser desorption/ionization time-of-flight mass spectrome
Aggregation and surface properties of F-specific RNA phages: Implication for membrane filtration processes
Keywords: PI، نقطه ایزوالکتریک; Isoelectric point; Aggregation; Surface properties; Bacteriophages; MembranePFU, plaque forming unit; pI, isoelectric point; μ, electrophoretic mobility; D, diffusion coefficient; DLS, dynamic light scattering
Beta-amylase degradation by serine endoproteinases from green barley malt
Keywords: PI، نقطه ایزوالکتریک; Beta-amylase; Proteolysis; Barley; MaltDTT, dithiothreitol; E-64, l-trans-epoxysuccinyl-leucylamide-(4-guanido)-butane; IEF, isoelectric focussing; MW, molecular weight; PAGE, polyacrylamide gel electrophoresis; pI, isoelectric point; PMSF, phenylmethylsu
Serum amyloid A isoforms in serum and synovial fluid from spontaneously diseased dogs with joint diseases or other conditions
Keywords: PI، نقطه ایزوالکتریک; pI, isoelectric point; SAA, serum amyloid A; SF, synovial fluidAcute phase proteins; Arthritis; Canine; Serum amyloid A; Isoforms; Isoelectric focusing
Protein-coated β-ferric hydrous oxide particles: An electrokinetic and electrooptic study
Keywords: PI، نقطه ایزوالکتریک; PC, plastocyanin; PSI, photosystem I; p⊥, permanent dipole moment; γǀǀ, electric polarizability; ue, electrophoretic mobility; pI, isoelectric point; Lr, hydrodynamic thicknessProtein adsorption; Plastocyanin isoforms; β-Ferric hydrous oxide particles; Su
Drosophila DJ-1 mutants are sensitive to oxidative stress and show reduced lifespan and motor deficits
Keywords: PI، نقطه ایزوالکتریک; PD, Parkinson's disease; DA, dopaminergic; pI, isoelectric point; SNc, susbtantia nigra pars compacta; DM; dorsomedial; JNK, Jun N-terminal Kinase.Parkinson's disease; Paraquat; Neurodegeneration; Climbing ability
Cloning and sequencing of the Bet v 1-homologous allergen Fra a 1 in strawberry (Fragaria ananassa) shows the presence of an intron and little variability in amino acid sequence
Keywords: PI، نقطه ایزوالکتریک; 2DE, 2-dimensional polyacrylamide gel electrophoresis; pI, isoelectric point; rMa l d 1, recombinantly expressed Mal d 1; rBet v 1, recombinantly expressed Bet v 1; His-rFra a 1, His-tagged recombinantly expressed Fra a 1; nFra a 1, native Fra a 1; Mal d
The Loxtox protein family in Loxosceles intermedia (Mello-Leitão) venom
Keywords: PI، نقطه ایزوالکتریک; Loxosceles intermedia; Venom; Dermonecrotic protein; LiD1; Loxtox; Sphingomyelinasebp, base pair(s); Da, Daltons; L. gaucho, Loxosceles gaucho; L. intermedia, Loxosceles intermedia; L. similis, Loxosceles similis; pI, isoelectric point; SMase D, sphingomy
A novel nodule-enhanced gene encoding a putative universal stress protein from Astragalus sinicus
Keywords: PI، نقطه ایزوالکتریک; MJ0577; Nitrogen fixation; Nodule-enhanced gene expression; Nodulin; Symbiosis; Universal stress proteinMw, molecular weight; NF, Nod factor; ORF, open reading frame; pI, isoelectric point; RACE, rapid amplification of cDNA ends; RT-PCR, reverse transcrip