کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2819446 1569930 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Drosophila DJ-1 mutants are sensitive to oxidative stress and show reduced lifespan and motor deficits
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Drosophila DJ-1 mutants are sensitive to oxidative stress and show reduced lifespan and motor deficits
چکیده انگلیسی

Parkinson's disease (PD) is a progressive movement disorder caused by the selective and massive loss of dopaminergic neurons (DA) in the substantia nigra pars compacta (SNc). DJ-1 loss-of-function mutations are involved in inherited early-onset PD forms and result in dysfunction of the oxidative stress response. In mice models, DJ-1 loss provokes sensitivity to oxidative insults but does not produce neurodegeneration. Similar results have been found when analyzing Drosophila mutants for the DJ-1 orthologous genes, DJ-1α and DJ-1β. Here, we report the analysis of two new mutations for the Drosophila DJ-1 genes. Both ubiquitous induction of DJ-1α knockdown by RNAi and loss of function of DJ-1β caused by an insertional mutation result in increased sensitivity to paraquat insults, reduced lifespan and motor impairments. However these mutations do not lead to DA neuron loss. Besides, we find that targeted inhibition of DJ-1α function in DA neuron results in certain DA neurodegeneration. Our results, together with findings in other Drosophila DJ-1 mutants, indicate that both Drosophila DJ-1 genes are implicated in the protection against the chemical induced oxidative stress response, but also in fly survival. The differences observed in DA neurodegeneration suggest that the motor impairments exhibited by the mutants could be caused by different pathways.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 400, Issues 1–2, 1 October 2007, Pages 158–165
نویسندگان
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