کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10078808 | 1603618 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Repair of Osteochondral Defect With Tissue-Engineered Chondral Plug in a Rabbit Model
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
ارتوپدی، پزشکی ورزشی و توانبخشی
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![عکس صفحه اول مقاله: Repair of Osteochondral Defect With Tissue-Engineered Chondral Plug in a Rabbit Model Repair of Osteochondral Defect With Tissue-Engineered Chondral Plug in a Rabbit Model](/preview/png/10078808.png)
چکیده انگلیسی
Purpose: The purpose of this study was to evaluate the macroscopic and histologic results of transplanting a tissue-engineered chondral plug made of atelocollagen sponge and PLLA mesh to treat osteochondral defects. Type of Study: Controlled experimental study. Methods: Twelve-week-old male Japanese white rabbits were used. Fresh articular cartilage slices were taken from the humeral head, and isolated chondrocytes were embedded in atelocollagen gel which does not have antigenic portions of collagen (2.0 à 106 cells/mL). They were seeded on the top of the atelocollagen sponge/PLLA mesh composite and cultured for 2 weeks. The culture medium was changed every 3 days and L-ascorbic acid (50 μg/mL) was added every 2 days. Culturing the composites for 2 weeks produced tissue-engineered chondral plugs. These tissue-engineered chondral plugs (4-mm diameter, 4-mm thick) were transplanted into the osteochondral defects (4 mm diameter, 4 mm deep) in the patellar grooves of the same rabbits from which the chondrocytes had been harvested (the experimental group). In the control group, the defects were treated with the plugs without chondrocytes. The rabbits were killed 4 and 12 weeks after transplantation. The repaired tissues were evaluated macroscopically and histologically, and analyzed immunohistochemically for expression of type II collagen. Results: Four weeks after transplantation in the experimental group, the defects were partially repaired with cartilage-like tissue with good subchondral bone formation. Twelve weeks after transplantation, the defects were repaired with hyaline cartilage-like tissue densely stained by Safranin O. Well-organized subchondral bone formation was also observed. In the control group, the defects were covered with only soft fibrous tissue at 4 and 12 weeks macroscopically. Immunohistochemically, type II collagen was detected in about 90% of the repaired area. Histologic scores in the experimental group were significantly higher than those in the control group at both 4 and 12 weeks after transplantation. Conclusions: This study shows that the defects treated with tissue engineered chondral plug developed type II collagen in about 90% of the repaired area. Clinical Relevance: The transplantation of a tissue-engineered chondral plug will be one option for treating osteochondral defects. The next step in testing our hypothesis is to evaluate the repaired tissue biomechanically and biochemically over a longer period of time.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Arthroscopy: The Journal of Arthroscopic & Related Surgery - Volume 21, Issue 10, October 2005, Pages 1155-1163
Journal: Arthroscopy: The Journal of Arthroscopic & Related Surgery - Volume 21, Issue 10, October 2005, Pages 1155-1163
نویسندگان
Yohei M.D., Mitsuo M.D., Nobuo M.D., Katsura Ph.D., Shinobu M.S., Yoshito Ph.D., Panjaphongse M.D.,