کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10154421 | 1666296 | 2019 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Co-localization of glucose oxidase and catalase enabled by a self-assembly approach: Matching between molecular dimensions and hierarchical pore sizes
ترجمه فارسی عنوان
هماهنگ سازی گلوکز اکسیداز و کاتالاز با استفاده از روش خودمراقبتی: تطابق بین ابعاد مولکولی و اندازه منافذ سلسله مراتبی
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کلمات کلیدی
ساختار پوسته، اسید گلوکونیک، ظرفیت جذب، رویکرد خودمراقبتی، بی حرکت سازی چند آنزیم،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
To achieve efficient one-step production of gluconic acid, cascade reactions of glucose oxidase (GOD) and catalase (CAT) have been advocated in the biocatalysis system. In this work, the methodology of co-immobilization of GOD and CAT was investigated in details for obtaining improved enzyme loading and activity. The maximum adsorption capability of GOD and CAT was 24.18 and 14.33â¯mg·gâ1, respectively. The matching between dimensions of enzymes and hierarchical pore sizes of carriers are critical to the success of immobilization process. The simultaneous self-assembly on glutaraldehyde cross-linked mesoporous carriers exhibited favorable properties in comparison with sequential immobilization of GOD and CAT. The conversion of glucose under adequate air by co-localized GOD&CAT sustained the activity more than 90% after repeated utilization in the production of sodium gluconate and gluconic acid, suggesting that the co-immobilized GOD&CAT could be a promising catalyst for gluconate and gluconic acid production in some chemical and food industries.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 275, 1 March 2019, Pages 197-205
Journal: Food Chemistry - Volume 275, 1 March 2019, Pages 197-205
نویسندگان
Wei Zhuang, Jinsha Huang, Xiaojing Liu, Lei Ge, Huanqing Niu, Zhenfu Wang, Jinglan Wu, Pengpeng Yang, Yong Chen, Hanjie Ying,