کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10163601 | 1144074 | 2016 | 35 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential Effects of Dexamethasone and Doxycycline on Inflammation and MMP Production in Murine Alkali-Burned Corneas Associated with Dry Eye
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کلمات کلیدی
BSSDOXYM-MuLVQIDDesiccating stressMMPDEXMmpsMPO - DFOdry eye - خشکی چشمdoxycycline - داکسی سایکلینUntreated - درمان نشدهDexamethasone - دگزامتازونdays - روزهاalkali burn - سوزاندن قلیاییCorticoid - قارچ خوراکیmatrix metalloproteinase - ماتریکس متالوپروتئینازbalanced salt solution - محلول نمک متعادلmyeloperoxidase - میلوپراکسیداز Neutrophils - نوتروفیل هاMoloney murine leukemia virus - ویروس لوئی موش MoloneyBID - پیشنهاد
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
چشم پزشکی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Alkali burns to the cornea are among the most devastating injuries to the eye. The purpose of this study was to evaluate the effects of dexamethasone (Dex) or doxycycline (Doxy) on protease activity and corneal complications in a combined model (CM) of alkali burn and dry eye. C57BL/6 mice were subjected to the CM for 2 or 5 days (D). Mice were topically treated either with Dex (0.1%), Dox (0.025%) or vehicle QID and observed daily for appearance of corneal perforation. Quantitative real time PCR was performed to measure expression of inflammation cytokines and matrix metalloproteinases (MMPs) in whole cornea lysates. No perforations were observed in the Dex-treated corneas. All wounds in Doxy-treated corneas were closed 2D post-injury, and they had significantly lower corneal opacity scores at days 4 and 5 post-injury compared to BSS treatment. Dex-treated corneas had the lowest corneal opacity scores. Dex treatment significantly decreased expression of IL-1β, IL-6, MMPs -1, -9, -13, and TIMP-1 after 2 days but increased levels of MMP-8, while Doxy treatment significantly decreased IL-1β, IL-6, MMP-8, and -9, compared to vehicle. Decreased MMP-1, -9 and -13 immunoreactivity and gelatinolytic activity were seen in corneas treated with Doxy and Dex compared to vehicle. Increased neutrophil infiltration and myeloperoxidase activity was noted in the vehicle group compared to Dex 2 days post-injury. These findings demonstrate that early initiation of anti-inflammatory therapy is very efficacious in preserving corneal clarity and facilitating wound healing, while modulating MMP production and suppressing neutrophil infiltration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Ocular Surface - Volume 14, Issue 2, April 2016, Pages 242-254
Journal: The Ocular Surface - Volume 14, Issue 2, April 2016, Pages 242-254
نویسندگان
Fang MD, PhD, Flavia S.A. MD, Johanna Tukler PhD, Stephen C. MD, Eugene A. MD, PhD, De-Quan MD, PhD, Cintia S. MD, PhD,