کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10163609 | 1144090 | 2013 | 17 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Tear Fluid Biomarker Profiling: A Review of Multiplex Bead Analysis
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
چشم پزشکی
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چکیده انگلیسی
Developments in the field of tear film protein profiling are reviewed, including the advantages and disadvantages of the multiplex bead array technique and its potential for identifying biomarkers of ocular surface disease. Commercial kits, which specifically employ 96-well plates with polystyrene microspheres and magnetic beads, and sensitivity variations between them are discussed. Modifications to protocols of these kits (which are designed primarily for larger sample volumes, such as blood and cell culture supernatants) may be necessary for tear fluid samples. Multiplex bead array is compared to the “gold standard,” ELISA. The challenges of diagnosing and monitoring dry eye disease, due to the conflicting and variable signs and symptoms presented, are illustrated by the authors' own study results. The development of a biomarker profile for the disease would be a useful approach to the ongoing problems of diagnosis. The multiplex bead array technique has important potential applications in this regard.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Ocular Surface - Volume 11, Issue 4, October 2013, Pages 219-235
Journal: The Ocular Surface - Volume 11, Issue 4, October 2013, Pages 219-235
نویسندگان
Suzanne PhD, Alan DSc, PhD, FCOptom,