کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10179982 1318700 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Transcriptome datasets supply basic gene information for RNAi pest management and gene functional studies in Nephotettix cincticeps (Uhler)
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Transcriptome datasets supply basic gene information for RNAi pest management and gene functional studies in Nephotettix cincticeps (Uhler)
چکیده انگلیسی
RNA interference (RNAi) technology has the potential to be used in pest management in crop production. Here, the transcriptome of Nephotettix cincticeps (Uhler) was deeply sequenced to investigate the systematic RNAi mechanism and candidate genes for dsRNA feeding. In our datasets, a total of 81 225 transcripts were obtained with the length from 150 bp to about 4.2 kb. Almost all the genes related to the RNAi core pathway were proved to be present in N. cincticeps transcriptome. Two transcripts that respectively encode a systemic interference defective (SID) were identified in our database, indicating that the systematic RNAi pathway can function effectively in N.cincticeps. Our datasets not only supply basic gene information for the studies of gene expression and functions in N. cincticeps, such as the control genes for gene expression analysis, but also provide candidate genes for RNAi pest management, such as the genes that encode P450 monooxygenase, V-ATPase and chitin synthase.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Integrative Agriculture - Volume 15, Issue 4, April 2016, Pages 840-847
نویسندگان
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