کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10227498 | 449 | 2014 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ontology analysis of global gene expression differences of human bone marrow stromal cells cultured on 3D scaffolds or 2D films
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Ontology analysis of global gene expression differences of human bone marrow stromal cells cultured on 3D scaffolds or 2D films Ontology analysis of global gene expression differences of human bone marrow stromal cells cultured on 3D scaffolds or 2D films](/preview/png/10227498.png)
چکیده انگلیسی
Differences in gene expression of human bone marrow stromal cells (hBMSCs) during culture in three-dimensional (3D) nanofiber scaffolds or on two-dimensional (2D) films were investigated via pathway analysis of microarray mRNA expression profiles. Previous work has shown that hBMSC culture in nanofiber scaffolds can induce osteogenic differentiation in the absence of osteogenic supplements (OS). Analysis using ontology databases revealed that nanofibers and OS regulated similar pathways and that both were enriched for TGF-β and cell-adhesion/ECM-receptor pathways. The most notable difference between the two was that nanofibers had stronger enrichment for cell-adhesion/ECM-receptor pathways. Comparison of nanofibers scaffolds with flat films yielded stronger differences in gene expression than comparison of nanofibers made from different polymers, suggesting that substrate structure had stronger effects on cell function than substrate polymer composition. These results demonstrate that physical (nanofibers) and biochemical (OS) signals regulate similar ontological pathways, suggesting that these cues use similar molecular mechanisms to control hBMSC differentiation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 35, Issue 25, August 2014, Pages 6716-6726
Journal: Biomaterials - Volume 35, Issue 25, August 2014, Pages 6716-6726
نویسندگان
Bryan A. Baker, P. Scott Pine, Kaushik Chatterjee, Girish Kumar, Nancy J. Lin, Jennifer H. McDaniel, Marc L. Salit, Carl G. Jr.,