کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10227573 449 2014 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Regulation of the endothelialization by human vascular endothelial cells by ZNF580 gene complexed with biodegradable microparticles
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Regulation of the endothelialization by human vascular endothelial cells by ZNF580 gene complexed with biodegradable microparticles
چکیده انگلیسی
Based on their positive charged surface, MPs can compact pEGFP-ZNF580 into MPs/pEGFP-ZNF580 complexes. The cell transfection result demonstrated that pEGFP-ZNF580 could be transported efficiently into ECs by these complexes. The result of western blot showed that the relative ZNF580 protein level can increase to 35.74%-46.11% by the overexpression of ZNF580 gene. Furthermore, the release of pEGFP-ZNF580 could be sustained at least 25 days due to the controllable degradation ability of the hydrophobic MPs' core. The MPs and MPs/pEGFP-ZNF580 complexes showed low cytotoxicity because of the introduction of PEG chains and low molecular weight PEI on the surface of these MPs. Notably, at the low concentration (20 μg/mL), the MPs and their complexes were non-cytotoxicity. The rapid endothelialization was promoted significantly by the expression of pEGFP-ZNF580.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 35, Issue 25, August 2014, Pages 7133-7145
نویسندگان
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