کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10227831 465 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Combining cell sheet technology and electrospun scaffolding for engineered tubular, aligned, and contractile blood vessels
ترجمه فارسی عنوان
ترکیبی از تکنولوژی ورق سلول و داربست الکترو اسپن برای رگ های خونی لوله ای، هم تراز و هماهنگ شده
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Herein we combine cell sheet technology and electrospun scaffolding to rapidly generate circumferentially aligned tubular constructs of human aortic smooth muscles cells with contractile gene expression for use as tissue engineered blood vessel media. Smooth muscle cells cultured on micropatterned and N-isopropylacrylamide-grafted (pNIPAm) polydimethylsiloxane (PDMS), a small portion of which was covered by aligned electrospun scaffolding, resulted in a single sheet of unidirectionally aligned cells. Upon cooling to room temperature, the scaffold, its adherent cells, and the remaining cell sheet detached and were collected on a mandrel to generating tubular constructs with circumferentially aligned smooth muscle cells which possess contractile gene expression and a single layer of electrospun scaffold as an analogue to a small diameter blood vessel's internal elastic lamina (IEL). This method improves cell sheet handling, results in rapid circumferential alignment of smooth muscle cells which immediately express contractile genes, and introduction of an analogue to small diameter blood vessel IEL.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 35, Issue 9, March 2014, Pages 2713-2719
نویسندگان
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