کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10227836 465 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Tuning the material-cytoskeleton crosstalk via nanoconfinement of focal adhesions
ترجمه فارسی عنوان
تنظیم تداخل ماده-سیتو اسکلت توسط نانوکامپوزیت چسبندگی کانونی
کلمات کلیدی
چسبندگی سلولی، مورفولوژی سلول، سلول پیش از قاعدگی، نانوذرات چسبنده، مکانیک انتقال، پلی متیل سیلوکسان،
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Material features proved to exert a potent influence on cell behaviour in terms of adhesion, migration and differentiation. In particular, biophysical and biochemical signals on material surfaces are able to affect focal adhesion distribution and cytoskeletal assemblies, which are known to regulate signalling pathways that ultimately influence cell fate and functions. However, a general, unifying model that correlates cytoskeletal-generated forces with genetic events has yet to be developed. Therefore, it is crucial to gain a better insight into the material-cytoskeleton crosstalk in order to design and fabricate biomaterials able to govern cell fate more accurately. In this work, we demonstrate that confining focal adhesion distribution and growth dramatically alters the cytoskeleton's structures and dynamics, which in turn dictate cellular and nuclear shape and polarization. MC3T3 preosteoblasts were cultivated on nanograted polydimethylsiloxane substrates and a thorough quantification - in static and dynamic modes - of the morphological and structural features of focal adhesions and cytoskeleton was performed. Nanoengineered surfaces provided well-defined zones for focal adhesions to form and grow. Unique cytoskeletal structures spontaneously assembled when focal adhesions were confined and, in fact, they proved to be very effective in deforming the nuclei. The results here presented provide elements to engineer surfaces apt to guide and control cell behaviour through the material-cytoskeleton-nucleus axis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 35, Issue 9, March 2014, Pages 2743-2751
نویسندگان
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