کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10228064 | 472 | 2014 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A fluorescent probe to detect thiol-containing amino acids in solid tumors
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Early detecting of cancer is critical to provide proper treatment and to improve survival of patients. Here, we reported a highly sensitive ratiometric (yellow emission (550Â nm) to blue emission (496Â nm)) fluorescent probe 1 developed for detection of thiol-containing amino acids. This probe successfully eliminates interference from background autofluorescence, and discriminates between human carcinoma and normal cells by detecting intracellular thiol levels in living cells (PÂ <Â 0.05). Furthermore, the ability of the probe to identify growing tumors by measuring GSH in the tissues as well as in the fresh blood of tumor xenograft mice. Additionally, the ratio of the emission intensity at two different wavelengths can provide quantitative analysis of glutathione (GSH) in the living systems. It suggests that it represents a promising prognostic and diagnostic marker, with extensive and simple potential clinical applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 35, Issue 13, April 2014, Pages 4157-4167
Journal: Biomaterials - Volume 35, Issue 13, April 2014, Pages 4157-4167
نویسندگان
Wen Xiu Ren, Jiyou Han, Tuhin Pradhan, Ja-Yun Lim, Jae Hong Lee, Jaehun Lee, Jong-Hoon Kim, Jong Seung Kim,