کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10228098 473 2013 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The regulation of growth and metabolism of kidney stem cells with regional specificity using extracellular matrix derived from kidney
ترجمه فارسی عنوان
تنظیم رشد و متابولیسم سلول های بنیادی کلیه با خاصیت منطقه ای با استفاده از ماتریکس خارج سلولی از کلیه حاصل می شود
کلمات کلیدی
ماتریکس غیر سلولی، هیدروژل، کلیه، سلول های بنیادی، موقعیت مناسب، جایگاه، ویژگی منطقه ای،
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Native extracellular matrix (ECM) that is secreted and maintained by resident cells is of great interest for cell culture and cell delivery. We hypothesized that specialized bioengineered niches for stem cells can be established using ECM-derived scaffolding materials. Kidney was selected as a model system because of the high regional diversification of renal tissue matrix. By preparing the ECM from three specialized regions of the kidney (cortex, medulla, and papilla; whole kidney, heart, and bladder as controls) in three forms: (i) intact sheets of decellularized ECM, (ii) ECM hydrogels, and (iii) solubilized ECM, we investigated how the structure and composition of ECM affect the function of kidney stem cells (with mesenchymal stem cells, MSCs, as controls). All three forms of the ECM regulated KSC function, with differential structural and compositional effects. KSCs cultured on papilla ECM consistently displayed lower proliferation, higher metabolic activity, and differences in cell morphology, alignment, and structure formation as compared to KSCs on cortex and medulla ECM, effects not observed in corresponding MSC cultures. These data suggest that tissue- and region-specific ECM can provide an effective substrate for in vitro studies of therapeutic stem cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 34, Issue 38, December 2013, Pages 9830-9841
نویسندگان
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