کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10228227 | 475 | 2013 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression of the α5 integrin gene in corneal epithelial cells cultured on tissue-engineered human extracellular matrices
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Expression of the α5 integrin gene in corneal epithelial cells cultured on tissue-engineered human extracellular matrices Expression of the α5 integrin gene in corneal epithelial cells cultured on tissue-engineered human extracellular matrices](/preview/png/10228227.png)
چکیده انگلیسی
The integrin α5β1 plays a major role in corneal wound healing by promoting epithelial cell adhesion and migration over the fibronectin matrix secreted as a cellular response to corneal damage. Expression of α5 is induced when rabbit corneal epithelial cells (RCECs) are grown in the presence of fibronectin. Here, we examined whether α5 expression is similarly altered when RCECs or human corneal epithelial cells (HCECs) are grown on a reconstructed stromal matrix used as an underlying biomaterial. Mass spectrometry and immunofluorescence analyses revealed that the biomaterial matrix produced by culturing human corneal fibroblasts with ascorbic acid (ECM/35d) contains several types of collagens, fibronectin, tenascin and proteoglycans. Results from transfection of CAT/α5-promoter plasmids, Western blot and EMSA analyses indicated that ECM/35d significantly increase expression of α5 in HCECs as a result of alteration in the expression and DNA binding of the transcription factors NFI, Sp1, AP-1 and PAX6. The biological significance of this biomaterial substitute on the expression of the α5 gene may therefore contribute to better understand the function played by the α5β1 integrin during corneal wound healing.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 34, Issue 27, September 2013, Pages 6367-6376
Journal: Biomaterials - Volume 34, Issue 27, September 2013, Pages 6367-6376
نویسندگان
Jennifer Lake, Karine Zaniolo, Manon Gaudreault, Patrick Carrier, Alexandre Deschambault, Richard Bazin, Lucie Germain, Christian Salesse, Sylvain L. Guérin,