کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10228439 | 482 | 2013 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The effect of adipose tissue derived MSCs delivered by a chemically defined carrier on full-thickness cutaneous wound healing
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کلمات کلیدی
α-SMAadipose tissue derived mesenchymal stem cellsPBSAT-MSCsTGF-β1PECAM-1CFSEβ2MGAPDHCFU-FPFARIPAradio-immunoprecipitation assay - آزمایش ادرار رادیو ایمنalpha smooth muscle actin - آلفا آکتیو عضله صافWound Healing - التیام زخمtransforming growth factor beta 1 - تبدیل فاکتور رشد بتا 1tumour necrosis factor-alpha - تومور نکروز عامل آلفاMesenchymal stem cells - سلول های بنیادی مزانشیمیVascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)TNF-α - فاکتور نکروز توموری آلفاPhosphate buffered saline - فسفات بافر شورplatelet endothelial cell adhesion molecule-1 - مولکول چسبندگی سلول اندوتلیال پلاکتی-1Beta-2 microglobulin - میکروگلوبولین بتا-2paraformaldehyde - پارافرمالدهیدcarboxyfluorescein diacetate succinimidyl ester - کربوکسیفلوورسسین دی سکته سوکسینیمیدیل استرglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژناز
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Mesenchymal stem cells (MSCs) have properties which make them promising for the treatment of chronic non-healing wounds. A major so far unmet challenge is the efficient, safe and painless delivery of MSCs to skin wounds. Recently, a surface carrier of medical-grade silicone coated by plasma polymerisation with a thin layer of acrylic acid (ppAAc) was developed, and shown to successfully deliver MSCs to deepithelialised human dermis in vitro. Here we studied the potential of the ppAAc carrier to deliver human adipose tissue derived MSCs (AT-MSCs) to murine full-thickness excisional skin wounds in vivo. Further we investigate the mechanism of action of MSCs in accelerating wound healing in these wounds. AT-MSCs cultured on ppAAc carriers for 4 days or longer fully retained their cell surface marker expression profile, colony-forming-, differentiation- and immunosuppressive potential. Importantly, AT-MSCs delivered to murine wounds by ppAAc carriers significantly accelerated wound healing, similar to AT-MSCs delivered by intradermal injection. More than 80% of AT-MSCs were transferred from carriers to wounds in 3 days. AT-MSCs were detectable in wounds for at least 5 days after wounding. Carrier delivered AT-MSCs were demonstrated to have the capacity to down-modulate TNF-α-dependent inflammation, increase anti-inflammatory M2 macrophage numbers, and induce TGF-β1-dependent angiogenesis, myofibroblast differentiation and granulation tissue formation, thereby enhancing overall tissue repair.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 34, Issue 10, March 2013, Pages 2501-2515
Journal: Biomaterials - Volume 34, Issue 10, March 2013, Pages 2501-2515
نویسندگان
Dongsheng Jiang, Yu Qi, Nathan G. Walker, Anca Sindrilaru, Adelheid Hainzl, Meinhard Wlaschek, Sheila MacNeil, Karin Scharffetter-Kochanek,