کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10228831 496 2012 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Selective stimulation of caveolae-mediated endocytosis by an osmotic polymannitol-based gene transporter
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Selective stimulation of caveolae-mediated endocytosis by an osmotic polymannitol-based gene transporter
چکیده انگلیسی
Controlling the cellular uptake mechanism and consequent intracellular route of polyplexes is important to improve the transfection efficiency of the non-viral gene delivery. Here, we report a new non-viral vector, polymannitol-based gene transporter (PMT), generated by crosslinking low molecular weight polyethylenimine with mannitol diacrylate, which has low cytotoxicity and good transfection efficiency. Interestingly, the uptake pathway of PMT/DNA complexes was shifted into caveolae-mediated endocytosis, avoiding lysosomal degradation. The mechanism of increased caveolae-mediated endocytosis of PMT/DNA complexes was found to be correlated with mechanosensing signal transduction by the hyperosmotic polymannitol part. Our results suggested that PMT, polymannitol-based gene transporter, is a safe and efficient gene delivery system with a well-modulated uptake pathway and intracellular route for gene therapy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 33, Issue 29, October 2012, Pages 7272-7281
نویسندگان
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