کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10229056 | 503 | 2013 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Supramolecular pseudo-block gene carriers based on bioreducible star polycations
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A series of supramolecular pseudo-block polycations (CD-SS-pDM/Ad-pPEGs) were realized by assembling bioreducible β-cyclodextrin-cored star poly (2-dimethyl amino)ethyl methacrylate with different molecular weight and an adamantine-ended linear poly(poly(ethylene glycol)ethyl ether methacrylate) (pPEGEEMA) via the host-guest interaction. The pseudo-block CD-SS-pDM/Ad-pPEG carriers were investigated in terms of DNA binding capability, cytotoxicity, gene transfection in HepG2 and COS7 cell lines, and in vivo anti-tumor activity. The pseudo-block carriers exhibited undiminished pDNA-condensing abilities compared with the starting star carriers. Meanwhile, the pseudo-block carriers displayed lower cytotoxicity and higher gene transfection efficiencies at various N/P ratios. These results are consistent with the favorable properties of pPEGEEMA as expected. Furthermore, cellular internalization results and in vivo anti-tumor activity analysis demonstrated that assembled pPEGEEMA could enhance the stability of pseudo-block carriers, thus improving their cellular internalization and gene transfection efficiency. The present study demonstrated that supramolecular pseudo-block polycations via the host-guest interaction is an effective means to produce new gene carriers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 34, Issue 21, July 2013, Pages 5411-5422
Journal: Biomaterials - Volume 34, Issue 21, July 2013, Pages 5411-5422
نویسندگان
Yang Hu, Wei Yuan, Na-Na Zhao, Jie Ma, Wan-Tai Yang, Fu-Jian Xu,