Improving alternate lignin catabolite utilization of LigAB from Sphingobium sp. strain SYK-6 through site directed mutagenesis

- Mutation of LigAB enhances use of lignin derived aromatic compounds.
- Multiple mutants have enhanced catalytic efficiency with 3OMG.
- F103H has enhanced catalytic efficiency for both 3OMG and GA.
- Phe103α LigAB mutants outperform native catalyst DesZ for 3OMG dioxygenation.