کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10235205 | 45021 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High-cell-density cultivation of an engineered Rhodococcus opacus strain for lipid production via co-fermentation of glucose and xylose
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: High-cell-density cultivation of an engineered Rhodococcus opacus strain for lipid production via co-fermentation of glucose and xylose High-cell-density cultivation of an engineered Rhodococcus opacus strain for lipid production via co-fermentation of glucose and xylose](/preview/png/10235205.png)
چکیده انگلیسی
An engineered strain MITXM-61 of Rhodococcus opacus PD630 capable of utilizing xylose was used to produce biofuel precursor-triacylglycerols (TAG) from mixed glucose-xylose substrates. Optimal nitrogen source and carbon source concentrations were investigated for cell growth and microbial lipid production by MITXM-61 in flask cultures. In a two-stage batch culture, the maximum lipid yield (0.152 g TAG per g consumed carbon source) was achieved by utilizing a mixture of glucose and xylose. The fed-batch culture featured intermittent feeding of refined xylose solution during the lipid accumulation stage provided 45 g Lâ1 of cell dry weight, 54% (g TAG per g cell dry weight) of lipid content, 0.179 g gâ1 (g TAG per g consumed carbon source) of lipid yield, and a 6.9 g Lâ1 dayâ1 of lipid productivity. Fatty acids extracted from microbial lipids produced by MITXM-61 were predominately palmitic and oleic acids, which are the major components in TAG-derived biofuels.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 50, Issue 4, April 2015, Pages 500-506
Journal: Process Biochemistry - Volume 50, Issue 4, April 2015, Pages 500-506
نویسندگان
Qiang Fei, Sandra J. Wewetzer, Kazuhiko Kurosawa, ChoKyun Rha, Anthony J. Sinskey,