Thermostable amidase catalyzed production of isonicotinic acid from isonicotinamide

- Biotransformation of isonicotinamide to isonicotinic acid at 1 L fed batch scale.
- Different process parameters were optimized for production of isonicotinic acid.
- Amidase of G. subterraneus RL-2a showed superior operational thermostability.
- Isonicotinic acid was produced (61.55 g) at a rate of 1.18 g h−1 g dcw−1.