Novel aqueous two-phase systems based on tetrahydrofuran and potassium phosphate buffer for purification of lipase

- Novel ATPS composed of THF and K2HPO4/KH2PO4 at pH 7 was studied.
- The phase diagram, tie-lines and critical point were determined at 298 K.
- The concentration of components and temperature of equilibrium was investigated.
- The enzyme partitioning is spontaneous and governed by entropic effects.
- Lipase from Bacillus produced by submerged fermentation was purified 103.9 (fold).