Evaluation of divinylsulfone activated agarose to immobilize lipases and to tune their catalytic properties

- DVS agarose bead is a very suitable support to immobilize TLL.
- The immobilization protocol is critical to have good enzyme properties.
- The enzyme may be immobilized at different pH values.
- Further incubation at pH 10 improves stability.
- The pH of the first immobilization determines the final biocatalyst properties.