At-line mid infrared spectroscopy for monitoring downstream processing unit operations

- Direct applicability of mid infrared spectroscopy to in situ samples of downstream processing is shown.
- Possibility to monitor different critical quality attributes.
- Applicable to different steps of purification cascade.
- Quantification possible down to: 1% [w/w] aggregates, 700 ng ml−1 HCP, 0.7 g L−1 mAb.