کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10235373 | 45031 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Predicting acidic and alkaline enzymes by incorporating the average chemical shift and gene ontology informations into the general form of Chou's PseAAC
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Knowledge of the adaptation mechanism of enzymes to extreme pH values and distinguishing them from one another are necessary in the proteomics field, and would help in the drug design of stable enzymes. In this work, we have systematically analyzed the information of 105 acidic and 112 alkaline enzymes, and propose an approach for distinguishing acidic enzymes from alkaline enzymes by combining the amino acid composition, reduced amino acid composition, gene ontology, evolutionary information, and auto covariance of averaged chemical shift (acACS). The overall prediction accuracy is 94.01% by 10-fold cross-validation using the algorithm of support vector machine. This result is better than that obtained by other existing methods. The improvement of the overall prediction accuracy reaches up to 3.3% higher than those of the random forest algorithm and secondary structure amino acid composition. The acACS performance is excellent, indicating that our approach is better than other existing methods in the literature. A user-friendly web-server pred-enzymes for predicting acidic and alkaline enzymes has been established, which is accessible to the public.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 48, Issue 7, July 2013, Pages 1048-1053
Journal: Process Biochemistry - Volume 48, Issue 7, July 2013, Pages 1048-1053
نویسندگان
Guo-Liang Fan, Qian-Zhong Li, Yong-Chun Zuo,