Extraction, isolation and analysis of chondroitin sulfate from broiler chicken biomass

► Isolation of glycosaminoglycans from broiler chicken by-product from mechanical deboning. ► Enzymatic hydrolysis with papain, pancreatin, kiwi fruits and flavourzyme. ► Purification of chondroitin sulfate with anion exchange chromatography and ethanol precipitation. ► Immunodiffusion analyses of keratan sulfate.