New biocatalytic route for the production of enantioenriched β-alanine derivatives starting from 5- and 6-monosubstituted dihydrouracils

► We show a mimetic enzymatic tandem for the production of enantioenriched β-amino acids. ► New insights into the enantioselectivity of N-carbamoyl-β-alanine amidohydrolase. ► Determination of the enantioselectivity of dihydropyrimidinase. ► Both enzymes proved to be efficient with concentrations of substrate up to 1 M.