Sequence analysis, cloning and extracellular expression of cyclodextrin glucanotransferase gene from the alkaliphilic Bacillus pseudalcaliphilus 8SB in Escherichia coli

► Cloning of a new cgt-gene from B. pseudalcaliphilus 8SB is reported. ► The present study is the first evidence for the use of pJET as expression vector. ► Efficient extracellular CGTase production by Escherichia coli recombinant is achieved.