A 24-microwell plate with improved mixing and scalable performance for high throughput cell cultures

► We report a novel 24-microwell plate (MWP) as a miniature cell culture device. ► Each microwell has a central static mixer for improved mixing and data quality. ► Gas permeable membranes reduced evaporation without affecting aeration. ► Scalable cell culture performance comparable to spinner flasks was obtained. ► This provides a low-cost cell culture platform for biopharmaceutical process development.